ArticleBMC plant biology2025
Genes involved in the regulation of alkaloid and flavonoid biosynthesis in different tissues of Sophora tonkinensis via transcriptomics and metabolomics.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Development of Chloroplast Microsatellite Markers and Assessment of Genetic Diversity and Population Structure ofCurrent issues in molecular biology · 2026Article
- Overproduction of tropane alkaloids in transgenic Hyoscyamus reticulatus hairy roots L. elicited by silicon dioxide nanoparticles and precursor feeding by phenylalanine and putrescine.Scientific reports · 2026Article
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10 authors.
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Abstract
backgroundSophora tonkinensis Gagnep is a significant Chinese herbal medicine, primarily composed of alkaloids and flavonoids, which are its key pharmacological components. Despite its importance, the metabolic pathways of these substances in S. tonkinensis remain inadequately explored.
resultsThis study investigates the molecular regulation of alkaloid and flavonoid accumulation in S. tonkinensis. Through high-throughput transcriptome sequencing (RNA-seq) and liquid chromatography-mass spectrometry (LC-MS) of seeds, leaves, stems, and roots, the research identifies differential metabolites and genes involved in alkaloid and flavonoid biosynthesis. The transcriptome analysis reveals 2,727 differentially expressed genes (DEGs), with 35 related to alkaloids and 48 to flavonoids. Metabolome analysis uncovers 296 differentially accumulated metabolites (DAMs), including 23 alkaloid-related DAMs and 23 flavonoid-related DAMs. Additionally, weighted gene co-expression network analysis suggests StCAO (evm. MODEL: 3.924) as a key regulator of alkaloid biosynthesis and StCHIs (evm. MODEL: 3.2047, evm. MODEL: 1.2104, and evm. MODEL: 1.2101) as crucial genes for flavonoid biosynthesis. To validate these findings, qPCR validation confirmed the consistency of expression trends for 12 selected DEGs across the roots, stems, leaves, and seeds of S. tonkinensis.
conclusionThis study offers a comprehensive analysis of the regulatory mechanisms governing alkaloid and flavonoid accumulation, as well as the associated key genes, across various S. tonkinensis tissues. These findings pave the way for future research into the regulatory processes of alkaloids and flavonoids in S. tonkinensis.
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