Evidence map›Paper›PMID 40604370›Full record

ArticleBMC plant biology2025

Comprehensive analysis of SPL gene family and miR156a/SPLs in the regulation of terpenoid indole alkaloid biosynthesis in Catharanthus roseus L.

Yaojie Zhang, Xiaofan Ni, Xueqing Fu, Ayat Taheri, Wei Zhang, Pin Liu, Hang Liu, Ling Li, Yuliang Wang, Kexuan Tang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. GhmiR156-International journal of molecular sciences · 2025
    Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yaojie ZhangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Xiaofan NiSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Xueqing FuSchool of Design, Shanghai Jiao Tong University, Shanghai, 200240, China.
Ayat TaheriSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Wei ZhangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Pin LiuSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Hang LiuSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Ling LiSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Yuliang WangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. wangyuliang@sjtu.edu.cn.
Kexuan TangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. kxtang@sjtu.edu.cn.

Funding

Gates Foundation INV-027291National Key Research and Development Program of China 2018YFA0900600, 2018National Key Research and Development Program of China 2023YFC3503901, 2023
6 · The paper itself

Abstract

backgroundCatharanthus roseus (C. roseus), belongs to the dogbane family Apocynaceae, is the source of various terpenoid indole alkaloids (TIAs) with excellent antitumor properties, including vinblastine, vincristine, ajmalicine, serpentine, vindoline, and catharanthine. However, TIAs are widely used in clinical anticancer research, their content is extremely low in plants, and currently insufficient to meet market demand. Therefore, exploring TIAs regulation is essential to improve TIAs yield. Numerous transcription factors have been shown to be involved in vinblastine biosynthesis, while the function of SPL members (CrSPL) has not yet been investigated in C. roseus. SPL genes regulate both plant growth and development as well as metabolite accumulation.

resultsIn this study, genome-wide analysis identified fourteen CrSPL genes. The analysis included their gene structure, phylogenetic relationships, spatio-temporal expression profiles, response to methyl jasmonate and microRNA interactions. The yeast-one-hybrid (Y1H) and Dual-Luciferase (Dual-Luc) assays and transient overexpression show that CrSPLs can directly regulate the vinblastine biosynthesis pathway. The Cro-microRNA member, Cro-miR156a, can efficiently inhibit the activity of CrSPLs members and TIAs pathway genes.

conclusionsCro-miR156a and CrSPLs regulate TIAs biosynthesis, and this study provides a promising reference for investigating the roles of CrSPL TFs in regulating TIAs biosynthesis in C. roseus.

Indexed as

CatharanthusMicroRNAsPlant ProteinsSecologanin Tryptamine AlkaloidsTranscription FactorsGene Expression Regulation, PlantGenes, PlantMultigene FamilyPhylogenyMicroRNAsPlant ProteinsSecologanin Tryptamine AlkaloidsTranscription FactorsCatharanthus roseusmicroRNA/SPLRegulationTerpenoid indole alkaloids

Identifiers

PMID40604370
PMCPMC12220395

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.