ArticleCell biochemistry and biophysics2025
Development of a Lemon-peppermint Essential Oil Nanoemulsion Blend: Effects on Bacillus spp. Inhibition, A549 Lung Cancer Cell Viability, Cell Cycle Progression, and Genotoxicity.
Article in Cell biochemistry and biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing resistance to treatments and limited effectiveness of singular medications against cancer and foodborne bacteria highlight the need for therapeutic synergy using multiple natural components. This study aimed to develop a blend nanoemulsion (NE) combining two plant essential oils, lemon (LEO) and peppermint (PEO), for anticancer and antibacterial testing. The chemical composition of PEO and LEO was analyzed by GC-MS, identifying D-limonene (77.89%) and menthol (56.63%) as their primary constituents. The NEs were prepared with droplet sizes of 57.2 (LEO), 104.2 (PEO), and 44.3 nm (blend), confirmed by FTIR. Cellular viability, DNA damage, and cell cycle progression in A549 lung cancer cells were assessed, showing a 50% reduction in viability after treatment with LEO (1.7 mg/mL), PEO (4.9 mg/mL), and the blend (2.5 mg/mL). Blend NE induced apoptosis and halted the S-phase of the cell cycle. Antibacterial activity was evaluated against Bacillus cereus, Bacillus subtilis, and Pseudomonas aeruginosa. Regarding MIC, blend NE (20 mg/mL) was more effective than LEO-NE (40 mg/mL) and PEO-NE (80 mg/mL) against B. subtilis. TEM analysis confirmed bacterial lysis, supporting the blend NE's superior antimicrobial effects. This synergy holds promise for enhanced cancer treatment and antibacterial applications.
Indexed as
Identifiers
40603762What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.