Evidence map›Paper›PMID 40603642›Full record

ArticleScientific reports2025

TFF3 initiates gastric intestinal metaplasia by activating JAK2 and STAT3 under high salt conditions.

Yanhong Wang, Feifei Qian, Baodong Shan, Li Zhang, Jie Zhong, Song Chen, Manman Yao, Shengnan Li, Xixiang Cao, Sujuan Fei and 1 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yanhong Wang *Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Huaihai West Road, Xuzhou, 221004, Jiang Su, China.
Feifei Qian *Graduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Baodong ShanGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Li ZhangGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Jie ZhongGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Song ChenGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Manman YaoGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Shengnan LiGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Xixiang CaoGraduate school, Xuzhou Medical University, Xuzhou, 221009, China.
Sujuan Fei *Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Huaihai West Road, Xuzhou, 221004, Jiang Su, China.
Xunlei Pang *Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Huaihai West Road, Xuzhou, 221004, Jiang Su, China. pangxunlei@163.com.

Funding

Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX25_3282the Key Development Project of Science and Technology Bureau in Xuzhou KC22233the Key Laboratory of Jiangsu Province Open project XZSYSKF2021029the Project supported by the Affiliated Hospital of Xuzhou Medical University 2023ZL19
6 · The paper itself

Abstract

The mechanism underlying the role of trefoil factor family 3 (TFF3) in intestinal metaplasia remains unclear. This study reveals the molecular mechanism by which TFF3, in the process of gastric mucosal epithelial cell intestinal metaplasia (IM) induced by high salt, activates the JAK2/STAT3/CDX2 pathway, providing a potential target for the occurrence of IM. An in vitro model of high salt-induced intestinal metaplasia was established using bioinformatics to screen the GEO dataset for significantly differentially expressed genes related to intestinal metaplasia. The gastric epithelial cell line GES-1 was cultured in high-salt medium, and changes in cell function and the expression of TFF3, JAK2, STAT3, and CDX2 were examined following TFF3 knockdown or overexpression. Subsequent experiments disrupted the TFF3-JAK2/STAT3-CDX2 pathway to assess its effects on gene expression and cell function. The expression of TFF3 is upregulated during intestinal metaplasia, which promotes cell proliferation and migration. TFF3 regulates the expression of JAK2, STAT3, and CDX2 and activates the JAK2/STAT3 pathway to induce CDX2 expression in gastric epithelial cells, leading to intestinal metaplasia. Functional assays revealed that the TFF3-JAK2/STAT3-CDX2 pathway enhances both cell proliferation and migration. TFF3 induces intestinal metaplasia in gastric epithelial cells through the JAK2/STAT3-CDX2 pathway, providing new insights into the underlying mechanism and therapeutic strategies for intestinal metaplasia.

Indexed as

Gastric MucosaJanus Kinase 2MetaplasiaSTAT3 Transcription FactorTrefoil Factor-3CDX2 Transcription FactorCell LineCell MovementCell ProliferationEpithelial CellsHumansSignal TransductionCDX2 protein, humanCDX2 Transcription FactorJAK2 protein, humanJanus Kinase 2STAT3 protein, humanSTAT3 Transcription FactorTFF3 protein, humanTrefoil Factor-3CDX2High saltIntestinal metaplasiaJAK2/STAT3TFF3

Identifiers

PMID40603642
PMCPMC12222537

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.