Evidence map›Paper›PMID 40603527›Full record

ArticleScientific reports2025

Effects of estrogen and mechanical loading on cultured cells derived from mandibular condylar cartilage.

Jin Tsuchida, Yuya Nakao, Koken Sato, Osamu Uehara, Itaru Mizoguchi, Yoshihiro Abiko, Masahiro Iijima

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jin TsuchidaDivision of Orthodontics and Dentofacial Orthopedics, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Yuya NakaoDivision of Orthodontics and Dentofacial Orthopedics, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan. y-nakao@hoku-iryo-u.ac.jp.
Koken SatoDivision of Orthodontics and Dentofacial Orthopedics, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Osamu UeharaDivision of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Itaru MizoguchiDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-Machi, Aoba-Ku, Sendai, 980-8575, Japan.
Yoshihiro AbikoDivision of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
Masahiro IijimaDivision of Orthodontics and Dentofacial Orthopedics, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Funding

Japan Society for the Promotion of Science JP23K09443
6 · The paper itself

Abstract

Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative condition influenced by mechanical loading and hormonal factors, particularly estrogen. Despite its established role in TMJ pathophysiology, the mechanisms underlying the effects of estrogen on joint cartilage remain unclear, with conflicting reports regarding its protective and destructive effects. This study aimed to investigate the molecular effects of estrogen and mechanical loading on cultured cells derived from mandibular condylar cartilage (MCC). The chondrogenic identity of cells was confirmed using quantitative reverse transcription PCR (qRT-PCR). The cells were then subjected to mechanical loading with or without 17β-estradiol treatment. RNA sequencing and bioinformatics analyses, including differential gene expression, Gene Ontology, and protein-protein interaction network analysis, identified extracellular matrix (ECM)-related pathways and hub genes, such as COL1A1, COL3A1, LAMC1, and LAMB1, as key mediators. Mechanical loading reduced the expression of ECM-related genes in MCC cells, whereas estrogen combined with loading significantly upregulated COL1A1 and COL3A1 expression, as confirmed by qRT-PCR and western blotting. This study demonstrated the effects of estrogen on mechanical loading using superficial condylar cells to elucidate the mechanisms of TMJ. Estrogen may be, at least in part, significantly involved in TMJ-OA via altering of the ECM levels such as collagen, laminin, MMPs, and ADAMTS.

Indexed as

Cartilage, ArticularEstradiolEstrogensMandibular CondyleAnimalsCells, CulturedChondrocytesCollagen Type ICollagen Type I, alpha 1 ChainExtracellular MatrixGene Expression RegulationHumansOsteoarthritisProtein Interaction MapsStress, MechanicalTemporomandibular JointCollagen Type ICollagen Type I, alpha 1 ChainEstradiolEstrogensEstrogenExtracellular matrixMandibular condylar cartilageMechanical loadingTemporomandibular joint osteoarthritis

Identifiers

PMID40603527
PMCPMC12223048

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.