Evidence map›Paper›PMID 40603432›Full record

ArticleScientific reports2025

Preservation of bronchial artery circulation on ex-vivo lung perfusion.

Aravind Krishnan, Moeed Fawad, Stefan Elde, Elbert Heng, Alyssa Garrison, Ashley Y Choi, Joseph Simmons, John P McNulty, Blaine Chadwick, Chawannuch Ruaengsri and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Aravind KrishnanDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Moeed FawadDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Stefan EldeDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Elbert HengDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Alyssa GarrisonDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Ashley Y ChoiDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Joseph SimmonsDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
John P McNultyDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Blaine ChadwickDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Chawannuch RuaengsriDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Y Joseph WooDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
John Ward MacArthurDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA.
Brandon A GuenthartDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, USA. guenthart@stanford.edu.

Funding

T32 Training Program in Mechanisms and Innovation in Vascular DiseaseT32HL098049 · NHLBI · STANFORD UNIVERSITY · PI Nicholas James Leeper, Philip S Tsao · 2010 to 2026
$6.3M
NHLBI NIH HHS T32 HL098049NIH HHS NHLBI 1T32HL098049
6 · The paper itself

Abstract

Lungs receive blood supply from the pulmonary and bronchial arteries. After transplantation, lungs uniquely suffer from ischemia and injury to the airways, in part due to the surgical sacrifice of the bronchial artery circulation during procurement and implantation. Ex-vivo lung perfusion (EVLP) offers a platform to rehabilitate and assess donor lungs; however, it traditionally excludes bronchial circulation. We hypothesized that bronchial artery perfusion during EVLP may enhance airway preservation. A dual-circulation EVLP platform was developed using swine donor lungs with preserved bronchial arteries. Blood was separately routed to the pulmonary artery and to the bronchial arteries. Laser speckle contrast imaging (LASCA) was performed pre- and post-initiation of bronchial perfusion, and perfusion units were compared within lungs. Endpoint lung function and tissue integrity were assessed via histology and TUNEL staining after 6 h of perfusion. Six porcine lungs were successfully mounted on the dual-circulation EVLP system. Bronchial perfusion significantly increased airway perfusion (p = 0.03), with trends toward improved parenchymal perfusion as well. Histology demonstrated preserved architecture and less necrosis in the airways of dually perfused lungs. TUNEL staining demonstrated significantly reduced bronchial epithelial apoptosis in dual-perfused lungs compared to PA-only perfusion (p < 0.001). This study demonstrates the feasibility of dual-circulation EVLP in a large animal model and reveals early protective effects of bronchial artery perfusion on airway tissues. These findings suggest that incorporating bronchial perfusion into EVLP platforms may mitigate early ischemic airway injury, a key contributor to post-transplant complications and chronic lung allograft dysfunction. Further investigation is warranted to assess functional outcomes and long-term benefits, and to evaluate integration with bronchial artery revascularization strategies.

Indexed as

Bronchial ArteriesLungLung TransplantationOrgan PreservationPerfusionAnimalsSwine

Identifiers

PMID40603432
PMCPMC12222659

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.