Evidence map›Paper›PMID 40601719›Full record

ArticlePloS one2025

Vertical transmission of Dengue virus type-3 and metagenomic virome profiles of Aedes aegypti mosquitoes collected in Kisumu, Kenya.

Tabitha Wanjiru, Wallace Bulimo, Solomon Langat, Johnson Kinyua, Nicholas Odemba, Santos Yalwala, David Oullo, Richard Ochieng, Francis Ngere, Gladys Kerich and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Tabitha WanjiruDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.ORCID https://orcid.org/0009-0002-3597-8001
Wallace BulimoCentre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.ORCID https://orcid.org/0000-0001-8475-0586
Solomon LangatCentre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
Johnson KinyuaDepartment of Biochemistry, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi, Kenya.
Nicholas OdembaDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Santos YalwalaDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
David OulloDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Richard OchiengDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Francis NgereDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Gladys KerichDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Janet AmbaleDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Eunice AchiengDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
David AbuomDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Timothy EgboDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Jaree JohnsonDepartment of Entomology, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.
Elly OjwangDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
John EadsDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Eric GargesDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.
Fredrick EyaseDepartment of Emerging Infectious Diseases, Walter Reed Army Institute of Research-Africa, Nairobi, Kenya.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aedes aegypti is the main vector of several arboviruses including chikungunya, dengue, yellow fever and Zika. Beyond arboviruses, Aedes aegypti harbours insect-specific viruses (ISVs), which can modulate mosquito's ability to transmit diseases by interfering with viral processes and triggering immune responses. Both arboviruses and ISVs can be transmitted vertically, where viruses are passed from parent to offspring. The lack of systematic molecular and entomological surveillance, has left the diversity of viruses in local Aedes aegypti populations largely unexplored. This study aimed to characterize the viromes of Aedes aegypti mosquitoes from Kisumu, Kenya, focusing on viral diversity. Immature larvae and pupae were collected from Jua Kali area in Kisumu, reared into adults, and subjected to viral isolation by cell culture and metagenomic next-generation sequencing. RNA extraction, library preparation, and Illumina MiSeq sequencing were performed on CPE positive pools and metagenomic superpools. Initial data analysis was conducted using the CZ-ID platform, with quality control applied using PrinseqLite v0.20.4 to filter low-quality reads and remove adapters. De novo sequence assembly was performed with MEGAHIT v1.2.9, followed by BLAST analysis. Phylogenetic relationships were analyzed using the Maximum Likelihood method. A total of 2,142 female Aedes aegypti, grouped into 86 pools and 4 superpools, were analyzed using cell culture and metagenomic next-generation sequencing respectively. Dengue virus type-3 was detected in one of the 86 pool. Additionally, a variety of ISVs were identified, including Iflaviruses related to Tesano Aedes Iflavirus (TeAV), Armigeres Iflavirus, and Negeviruses related to Rabai Virus. An unclassified virus closely related to Korle-Bu Aedes virus was also detected. Our study provides insights into the viral diversity within Aedes aegypti mosquitoes in Kisumu and evidence of natural vertical transmission, specifically transovarial transmission of dengue virus type-3. Ongoing research is imperative to unravel vertical transmission mechanisms and subtleties governing ISV-arbovirus interactions across diverse environmental settings.

Indexed as

AedesDengue VirusInfectious Disease Transmission, VerticalMosquito VectorsViromeAnimalsDengueFemaleHigh-Throughput Nucleotide SequencingKenyaMetagenomeMetagenomicsPhylogeny

Identifiers

PMID40601719
PMCPMC12221173

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.