ArticlePloS one2025
Genome-wide identification and in-silico characterization of phytopathogenic Taf14 gene in Fusarium oxysporum fungus, and fungicide repurposing.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhytopathogenic fungi cause global agricultural damage of around 40%, for which there is an economic loss of more than $200 billion annually. F. oxysporum is one of the top five phytopathogenic fungi worldwide, which is extremely resistant to high temperatures and antiseptic chemicals. Therefore, it is crucial to find alternative fungicides against the F. oxysporum (Fo) pathogenic fungus in order to increase crop yields. The TATA box-binding protein (TBP)-associated factor 14 (Taf14) gene in Botrytis cinerea represented by (BcTaf14) is considered as the disease-causing gene in crops. Therefore, Taf14 protein has been recommended as the potential receptor to explore effective fungicides against the corresponding diseases in crops. However, the Taf14 gene in F. oxysporum is not yet identified and characterized.
objectivesThe specific objectives of this study are to (i) identify and characterize F. oxysporum Taf14 (FoTaf14) gene as the Fusarium wilt disease causing gene in crops, and (ii) exploring FoTaf14 gene guided candidate fungicides against the diseases in crops.
methodsThis study identified FoTaf14 proteins from the whole proteome of F. oxysporum by using BcTaf14 as the query protein in BLASTP search. The integrated bioinformatics studies including phylogenetic tree, domain analysis, subcellular location, pathway terms, regulatory elements and expression analysis were performed in order to characterize FoTaf14 proteins. Then we explored FoTaf14 proteins guided candidate fungicides against the Fusarium wilt disease in crops by molecular docking analysis.
resultsThe phylogenetic tree has shown that FoTaf14 and Taf14 from other fungal divisions are similar. The YEATS domain has been detected by domain analysis as having a direct impact on fungal pathogenicity. The subcellular location analysis revealed that all FoTaf14s are found in the nucleus and cytoplasm, which are directly related to fungal growth and pathogenicity. The gene ontology (GO) enrichment analysis has revealed some important GO-terms that are related to plant diseases. The cis-regulatory network analysis of FoTaf14 genes has shown that C-box, MYB, ABRE, SEF4, and TAAAG motifs are associated with stress, growth, and development, whereas GATA box, GT-1 motif, SEBF, HD, and W box are associated with virulence. Expression analysis has shown that FoTaf14 genes are highly expressed in the pathogenicity, growth, and development of F. oxysporum. Finally, we detected FoTaf14-protein-guided three repurposed fungicides (Oxathiapiprolin, Tolfenpyrad and Amisulbrom) for the treatment against the Fusarium wilt disease in crops by molecular docking analysis.
conclusionTherefore, the findings of this study might be useful resources for taking a proper treatment plan against Fusarium wilt disease to increase quality crop production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.