Evidence map›Paper›PMID 40601281›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Methods to Determine Oxidative Stress in Spermatozoa.

Jacinta H Martin, Leah Calvert, Amanda L Anderson, Tessa Lord, John E Schjenken, Natalie A Trigg, Geoffry N De Iuliis, Brett Nixon

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jacinta H MartinSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia. jacinta.martin@newcastle.edu.au.
Leah CalvertSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Amanda L AndersonSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Tessa LordSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
John E SchjenkenSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Natalie A TriggSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Geoffry N De IuliisSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.
Brett NixonSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Assessing oxidative stress levels in spermatozoa provides crucial information on the health of the cell, while also potentially providing insight into the systemic health of the individual. Indeed, elevated oxidative stress in spermatozoa has been implicated in compromising sperm function and, consequently, is now considered one of the leading mediators of numerous male reproductive pathologies. Here we describe two flow cytometric assays: Dihydroethidium and Mitosox Red, which can be used to detect reactive oxygen species originating from the cytosol and mitochondria, respectively, in both mouse and human spermatozoa. Such tools have utility in studies investigating the impact of reproductive aging, toxicants, and environmental exposures on the male germline. These assays are also beneficial in the context of testing novel pharmaceutical interventions or antioxidant defence therapies aimed at preserving fertility or for use in assisted reproduction technologies.

Indexed as

Flow CytometryOxidative StressSpermatozoaAnimalsCytosolEthidiumHumansMaleMiceMitochondriaPhenanthridinesReactive Oxygen SpeciesdihydroethidiumEthidiumPhenanthridinesReactive Oxygen SpeciesCytosolic reactive oxygen speciesDihydroethidium (DHE)Flow cytometryHumanMitochondrial reactive oxygen speciesMitosox Red (MSR)Mouse/miceOxidative stressReactive oxygen species (ROS)Spermatozoa/spermSytox® Green (SyG)

Identifiers

PMID40601281

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.