Evidence map›Paper›PMID 40601272›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Inhibiting Aldehyde Dehydrogenase Function Using WIN 18,446 to Synchronize Spermatogenesis.

Shelby L Havel, Michael D Griswold

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Shelby L HavelSchool of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Michael D GriswoldSchool of Molecular Biosciences, Washington State University, Pullman, WA, USA. mgriswold@wsu.edu.

Funding

Hormonal Regulation Sertoli Cell MaturationR01HD010808 · NICHD · WASHINGTON STATE UNIVERSITY · PI MICHAEL D GRISWOLD · 1985 to 2026
$6.0M
NICHD NIH HHS R01 HD010808
6 · The paper itself

Abstract

While beneficial to the individual, the asynchronous progression of spermatogenesis makes the evaluation of stage-specific or cell-specific function within the testes challenging for researchers. Here, we describe a method using the aldehyde dehydrogenase inhibitor WIN 18,446 to halt all-trans retinoic acid (atRA) synthesis and prevent the commitment of undifferentiated spermatogonia to meiosis. Following treatment with exogenous atRA after WIN 18,446, individual stages of spermatogenesis can be analyzed with high specificity and this method serves as an invaluable tool to study the progression of spermatogenesis.

Indexed as

Aldehyde DehydrogenaseEnzyme InhibitorsNaphthalenesSpermatogenesisAnimalsCell DifferentiationMaleMeiosisMiceSpermatogoniaTestisTretinoinAldehyde DehydrogenaseEnzyme InhibitorsNaphthalenesTretinoinAldehyde dehydrogenasesGerm cellsRetinoic acidRetinoidsSeminiferous epitheliumSertoli cellsSpermatogenesis

Identifiers

PMID40601272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.