Evidence map›Paper›PMID 40601142›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

On-Array Citrullination of Protein Microarrays.

Jacob Skallerup, Thomas B G Poulsen, Allan Stensballe

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jacob SkallerupDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Thomas B G PoulsenDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Allan StensballeDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark. as@hst.aau.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein microarrays are a powerful tool for investigating autoantigens that drive autoimmune diseases. However, in many cases, autoantibody reactivity is directed against slightly modified proteins, generating neo-autoantigens, rather than targeting native antigens. Citrullination, a post-translational modification, is a key example, playing a central role in the pathogenesis of rheumatoid arthritis (RA). Excessive activity of peptidylarginine deiminases (PAD) leads to the formation of neo-autoantigens that trigger an autoimmune response. This chapter describes a detailed protocol for on-array citrullination of protein microarrays. By incubating the arrays with a PAD-containing buffer, the protocol attempts to replicate the formation of citrullinated autoantigens that occurs in vivo and contributes to RA. This approach enables high-throughput identification of citrullinated autoantigens, providing a platform to identify novel autoantigens and explore their role in RA pathology.

Indexed as

AutoantigensCitrullinationCitrullineProtein Array AnalysisArthritis, RheumatoidAutoantibodiesHumansProtein-Arginine DeiminasesProtein Processing, Post-TranslationalAutoantibodiesAutoantigensCitrullineProtein-Arginine DeiminasesAnti-citrullinated protein antibody (ACPA)Autoantibody profilingCitrullinationCitrullineMicroarrayNeo-antigenNeo-autoantigenPeptidylarginine deiminase (PAD)Protein microarrayRheumatoid arthritis

Identifiers

PMID40601142

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.