ArticleMethods in molecular biology (Clifton, N.J.)2025
Bead-Based Suspension Array Using Peptides as Antigens to Capture and Identify Serum Antibodies.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Bead-based suspension arrays built with multiple antigens enable detection and identification of specific antibodies in human and animal serum. Here we describe how to use synthetic peptides as antigens in a serological Luminex assay. Biotinylated peptides immobilized on avidin beads are used to capture serum antibodies, which in turn are detected using a generic immunoglobulin-binding protein or broad anti-bird antibodies in resp. mammalian and avian sera. Background is suppressed by pre-blocking sera and by using a blocking agent during antibody capture. By employing an internal negative control, serum-specific cutoffs can be determined and/or signals can be normalized. The provided protocols allow creating multiplex bead-based suspension arrays using peptides as antigens to capture and detect specific serum antibodies from mammalians, including humans, and avian species.
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