Evidence map›Paper›PMID 40600531›Full record

ArticleCardiovascular & hematological disorders drug targets2025

Reduced Erythrocyte Opsonization by Calreticulin, Lactadherin, Mannose-binding Lectin, and Thrombospondin-1 in MAFLD Patients.

Zoi Kyriakou, Konstantinos Mimidis, Nikolaos Politis, Panagiotis Veniamis, Dimitris Vlachos, Konstantinos Anagnostopoulos, Charalampos Papadopoulos

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Article in Cardiovascular & hematological disorders drug targets, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zoi KyriakouLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Konstantinos Mimidis1st internal Medicine Clinic, University Hospital of Alexandroupolis, Alexandroupolis, Greece.
Nikolaos PolitisLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Panagiotis VeniamisLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Dimitris VlachosLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Konstantinos AnagnostopoulosLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Charalampos PapadopoulosLaboratory of Biochemistry, Department of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMetabolism dysfunction associated with fatty liver disease During metabolic hepatic inflammation (MAFLD), is characterized by systemic metabolism deregulation leading to increased hepatic erythrophagocytosis and subsequent iron overload and ferroptosis. Studies in animal models have shown that erythrocyte phosphatidylserine exposure drives erythrophagocytosis. However, the mechanism of erythrophagocytosis in human MAFLD has not been fully elucidated yet. Therefore, in this study, we explored the opsonins recognizing phosphatidylserine. In particular, we measured the levels of erythrocyte calreticulin, lactadherin, mannose-binding lectin, and thrombospondin-1.

methodsTwenty-four patients (15 men and 9 women) with MAFLD and 9 healthy controls (4 men and 5 women) were enrolled. Erythrocytes were isolated from EDTA-containing blood through multiple centrifugations and isotonic buffer. Protein levels were measured in erythrocyte lysates (triton X-100 0.1% v/v) or plasma with enzyme-linked immunosorbent assays.

resultsErythrocyte TSP-1 levels were reduced in MAFLD patients. This reduction was not followed by changes in plasma TSP-1 levels or erythrocyte calreticulin, lactadherin, and mannose- binding protein. DISCUSSION: Our results suggest that erythrophagocytosis in human MALFD, unlike animal models, is not mediated by opsonization of exposed phosphatidylserine.

conclusionOur study underlines the need for disease models that could better reflect the molecular pathogenesis of human MAFLD.

Indexed as

Antigens, SurfaceCalreticulinErythrocytesFatty LiverMannose-Binding LectinMilk ProteinsOpsonin ProteinsThrombospondin 1AdultFemaleHumansMaleMiddle AgedAntigens, SurfaceCalreticulinMannose-Binding LectinMFGE8 protein, humanMilk ProteinsOpsonin ProteinsThrombospondin 1Erythrocyteerythrophagocytosis.immunometabolismMAFLDopsonizationthrombospondin

Identifiers

PMID40600531

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.