Evidence map›Paper›PMID 40600291›Full record

ArticleLab on a chip2025

A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test.

Nicolas Castaño, Kaiser Chua, An Nguyen, Wyeth Marshall, Grady H Hofmann, Jonathan Lee, Mindy Tsai, Sayantani B Sindher, Kari C Nadeau, R Sharon Chinthrajah and 2 more

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In one paragraph

Article in Lab on a chip, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

12 authors.

Nicolas CastañoDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
Kaiser ChuaDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
An NguyenDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
Wyeth MarshallDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
Grady H HofmannDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
Jonathan LeeDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.
Mindy TsaiDepartment of Pathology, Stanford University, USA.
Sayantani B SindherSean N. Parker Center for Allergy and Asthma Research, Stanford University, USA.
Kari C NadeauSean N. Parker Center for Allergy and Asthma Research, Stanford University, USA.
R Sharon ChinthrajahSean N. Parker Center for Allergy and Asthma Research, Stanford University, USA.
Stephen J GalliDepartment of Pathology, Stanford University, USA.
Sindy K Y TangDepartment of Mechanical Engineering, Stanford University, USA. sindy@stanford.edu.ORCID 0000-0003-1465-2432

Funding

Tracking allergen specific T cells in multi-food allergyU19AI104209 · NIAID · STANFORD UNIVERSITY · PI GALLI, STEPHEN JOSEPH · 2013 to 2025
$17.3M
Probing basophil function in microfluidic systems for allergic disease diagnosisR21EB030643 · NIBIB · STANFORD UNIVERSITY · PI TANG, SINDY KAM-YAN · 2021 to 2023
$605k
An Integrated Micro-Basophil Activation Test for Rapid Food Allergy DiagnosticsR21AI149277 · NIAID · STANFORD UNIVERSITY · PI TANG, SINDY KAM-YAN · 2020 to 2021
$409k
NIAID NIH HHS R21 AI149277NIAID NIH HHS U19 AI104209NIBIB NIH HHS R21 EB030643
6 · The paper itself

Abstract

Access to the basophil activation test (BAT) has been hindered by the requirement for fresh blood analysis, specialized laboratory equipment, and advanced technical expertise. To address these issues, we have developed a hand-operated microfluidic sample preparation "μF-prep" device to perform the most time sensitive steps of the assay and stabilize the sample, effectively extending the time window before flow cytometry analysis. The μF-prep device performs concurrent basophil stimulation and staining for eight conditions in parallel. Barcoded staining of basophils allows the pooling of all conditions into one lyse/fix buffer tube for sample stabilization. After flow cytometry analysis, an XGBoost-enabled analysis pipeline unpools the eight conditions and generates basophil counts and activation levels directly from raw flow cytometry data. To characterize the μF-prep device, we stimulate whole blood samples from peanut-allergic and non-allergic donors. We compare μF-prep with a conventional BAT sample preparation protocol ("conv-prep"), and assess the stability of stimulated samples stored in the lyse/fix buffer. The μF-prep device performs sample preparation with <2 minutes of active user engagement. Our analysis pipeline shows excellent agreement with manual gating analysis. Compared with conv-prep, μF-prep exhibits similar activation levels at peanut doses of 1-100 ng mL

Indexed as

BasophilsLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesFlow CytometryHumansWorkflow

Identifiers

PMID40600291
PMCPMC12315523

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.