Evidence map›Paper›PMID 40600000›Full record

ArticleJournal of molecular and cellular cardiology plus2025

Pericellular oxygen dynamics in human cardiac fibroblasts and iPSC-cardiomyocytes in high-throughput plates: insights from experiments and modeling.

Weizhen Li, David McLeod, Sarah Antonevich, Maria R Pozo, Zhenyu Li, Emilia Entcheva

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular and cellular cardiology plus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Weizhen LiDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.
David McLeodDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.
Sarah AntonevichDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.
Maria R PozoDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.
Zhenyu LiDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.
Emilia EntchevaDepartment of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adequate oxygen supply is crucial for proper cellular function. The emergence of high-throughput (HT) expansion of human stem-cell-derived cells and HT in vitro cellular assays for drug testing necessitate monitoring and understanding of the oxygenation conditions, yet virtually no data exists for such settings. We used HT label-free optical measurements and computational modeling to gain insights about oxygen availability (pericellular oxygen dynamics) in syncytia of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) and human cardiac fibroblasts (cFB) grown in glass-bottom 96-well plates under static conditions. Our experimental results highlight the critical role of cell density and solution height (oxygen delivery path) in pericellular oxygen dynamics. The developed computational model, trained on the obtained comprehensive data set, revealed that time-variant maximum oxygen consumption rate, V

Indexed as

Cell cultureHigh-throughput screeningHuman cardiac fibroblastsiPSC-cardiomyocytesMichaelis-Menten modelModelingOxygen diffusionPericellular oxygen

Identifiers

PMID40600000
PMCPMC12212277

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.