Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Nicholas GaoDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.ORCID 0000-0003-0115-2447
Daniele MazzolettiDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.ORCID 0000-0002-0711-8269
Adele PengDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Paul Dominic B OlinaresLaboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10065, United States.ORCID 0000-0002-3429-6618
Castrese MorroneDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.
Andrea GaravagliaDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.
Nourelhoda GoudaDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Sergey TsoyDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.ORCID 0009-0000-8470-7270
Albert MendozaDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Ahammad ChowdhuryDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Antonio CerulloDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Hrutvik BhavsarDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.
Franca RossiDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.ORCID 0000-0001-7836-9500
Menico RizziDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.ORCID 0000-0002-7930-399X
Brian T ChaitLaboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10065, United States.ORCID 0000-0003-3524-557X
Riccardo MiggianoDepartment of Pharmaceutical Sciences, University of Piemonte Orientale, Novara 28100, Italy.ORCID 0000-0002-2159-3314
David JeruzalmiDepartment of Chemistry and Biochemistry, City College of New York, New York, NY 10031, United States.ORCID 0000-0001-5886-1370
Funding
TR&D Project 4. The Imaging Stage: Multiscale Spatiotemporal Modeling of Macromolecular Systems in Cellular NeighborhoodsP41GM109824 · NIGMS · ROCKEFELLER UNIVERSITY · PI ROUT, MICHAEL P · 2014 to 2023
$18.8M
TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
National Resource for Mass Spectrom of Biological MacromoleculesP41GM103314 · NIGMS · ROCKEFELLER UNIVERSITY · PI CHAIT, BRIAN T · 2012 to 2017
$9.9M
Dual-Beam Scanning Electron Microscope for New York Structural Biology CenterS10RR029300 · NCRR · NEW YORK STRUCTURAL BIOLOGY CENTER · PI STOKES, DAVID L. · 2010 to 2010
$2.0M
Acquisition of Detector for JEOL 3200 FSC Electron MicroscopeS10OD019994 · OD · NEW YORK STRUCTURAL BIOLOGY CENTER · PI CARRAGHER, BRIDGET OLIVIA · 2015 to 2015
$600k
Agouron Institute F00316Department of Education PA200A150068Fondazione Cariplo #2020-3589Italian Ministry of University and Research #2022LCN738Italian Ministry of University and Research PRIN 2022National Science Foundation MCB 1818255NCRR NIH HHS S10 RR029300NIGMS NIH HHS GM103310NIGMS NIH HHS P41 GM103310NIGMS NIH HHS P41 GM103314NIGMS NIH HHS P41 GM109824NIH HHS OD019994NIH HHS P41 GM103314NIH HHS P41 GM109824NIH HHS RR029300NIH HHS S10 OD019994NYSTARSimons Electron Microscopy Center and National Resource for Automated Molecular MicroscopySimons Foundation SF349247
6 · The paper itself
Abstract
Replicative helicases are assembled on chromosomes by helicase loaders before the initiation of DNA replication. Here, we investigate the mechanisms employed by the bacterial Vibrio cholerae (Vc) DnaB replicative helicase and the DciA helicase loader. Structural analysis of the ATPγS form of the VcDnaB-ssDNA complex reveals a configuration distinct from that observed with GDP•AlF4. With ATPγS, the amino-terminal domain (NTD) tier, previously found as an open spiral in the GDP•AlF4 complex, adopts a closed planar arrangement. Furthermore, the DnaB subunit at the top of the carboxy-terminal domain (CTD) spiral is displaced by approximately 25 Å between the two forms. We suggest that remodeling the NTD layer between closed planar and open spiral configurations, along with the migration of two distinct CTDs to the top of the DnaB spiral, repeated three times, mediates hand-over-hand translocation. Biochemical analysis indicates that VcDciA utilizes its Lasso domain to interact with DnaB near its Docking-Helix Linker-Helix interface. Up to three copies of VcDciA bind to VcDnaB, suppressing its ATPase activity during loading onto physiological DNA substrates. Our data suggest that DciA loads DnaB onto DNA using the ring-opening mechanism.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
DnaB and DciA: mechanisms of helicase loading and translocation on ssDNA. · full record | OpenQuestion