Evidence map›Paper›PMID 40598825›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Single-Cell Transcriptome Profiling Reveals Conserved IFNγ-IL8 Signaling-Induced Antibacterial Neutrophil States during Bacterial Infection.

Xue Zhai, Minghao Zhang, Kang Li, Wei Li, Xiaolong Li, Wa Gao, Zhaosheng Sun, Dan Huang, Songqian Huang, Mingli Liu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Xue ZhaiKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Minghao ZhangKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Kang LiState Key Laboratory of Estuarine and Coastal Research School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Wei LiKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Xiaolong LiThe State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, 730020, China.
Wa GaoKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Zhaosheng SunKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Dan HuangKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Songqian HuangKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Mingli LiuKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Zhichao WuKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Jun ZouKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Liangbiao ChenKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Jialong YangState Key Laboratory of Estuarine and Coastal Research School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Peng HuKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.ORCID https://orcid.org/0009-0002-6840-7758

Funding

National Natural Science Foundation of China 32200414 to P.HNational Natural Science Foundation of China 32341061National Natural Science Foundation of China 32373113
6 · The paper itself

Abstract

Streptococcus agalactiae is a significant pathogen in both humans and animals, yet the immune cell subtype dynamics during infection remain poorly defined. Leveraging the high susceptibility and tractability of Nile tilapia (Oreochromis niloticus), 113,356 single immune cells are profiled from head kidney and spleen across multiple infection time points (0, 1, 5, 10, 75 days post-infection and 3 days post-reinfection). This single-cell transcriptomic and flow cytometry analyses revealed distinct activation and transition patterns among neutrophils, macrophages, T cells, and B cells. Neutrophils exhibited early transcriptional remodeling enriched in inflammatory and interferon gamma (IFNγ) signaling pathways. Cross-species integration identified a conserved IFNγ-driven transition toward il8⁺ neutrophils. Furthermore, recombinant interleukin-8 (IL8) enhanced antibacterial responses in tilapia and human neutrophils, while inhibition of STAT1 reduced IL8 expression. IL8 stimulation increased phagocytosis and  reactive oxygen species (ROS） production, supporting its role in neutrophil-mediated bacterial clearance. Together, this findings establish IFNγ-IL8 as a conserved mechanism in vertebrate immunity and a potential target for antibacterial therapies.

Indexed as

Fish DiseasesInterferon-gammaInterleukin-8NeutrophilsStreptococcal InfectionsAnimalsCichlidsGene Expression ProfilingHumansSignal TransductionSingle-Cell AnalysisStreptococcus agalactiaeTranscriptomeInterferon-gammaInterleukin-8IFNγ‐IL8 signalingimmune responseneutrophilspublicly accessible datasetsingle‐cell transcriptomic profiles

Identifiers

PMID40598825
PMCPMC12499466

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.