Evidence map›Paper›PMID 40598498›Full record

ArticleCell communication and signaling : CCS2025

The Drosophila histone variant H2Av facilitates Notch signaling activity in a two-tier regulatory fashion.

Yao Chen, Xinyue Zhang, Yu Song, Jie Shen, Junzheng Zhang

Abstract read
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In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. In thebioRxiv : the preprint server for biology · 2026
    Article
4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Yao ChenState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Xinyue ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Yu SongState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Jie ShenState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Junzheng ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China. zhangjz@cau.edu.cn.

Funding

Beijing Natural Science Foundation 6242020National Natural Science Foundation of China 31970478
6 · The paper itself

Abstract

backgroundH2Av is an evolutionarily conserved H2A variant protein involved in the regulation of transcription. The Tip60 complex is recruited by different transcription factors to facilitate the incorporation and acetylation of H2Av, thereby influencing target gene expression. The Tip60-H2Av axis is involved in various developmental processes, though its precise roles are not yet fully understood.

methodsRNA interference and gene mutation technology were used to screen essential genes in regulating Notch signaling pathway. Immunostaining was used to detect the protein level of H2Av, Tip60 complex as well as Notch signaling pathway components. Chromatin immunoprecipitation assays were performed to detect the specific binding site of H2Av in E(spl)-Complex and Su(H) genes.

resultHere we report that H2Av is required for Notch signaling activation during Drosophila wing development. H2Av depletion disrupts the expression of Notch target genes, resulting in wing marginal defects. Unexpectedly, we find that H2Av regulates the expression of the Su(H) gene which encodes for the transcription factor of the Notch signaling cascade. We further demonstrate that the Tip60 complex modulates the transcription of both Notch targets and Su(H) likely through H2Av. Based on these observations, we propose a model that the Tip60-H2Av axis facilitates Notch pathway activation by simultaneously promoting the expression of both the target genes and the transcription factor.

conclusionThis study offers insights into the diverse roles of the Tip60-H2Av axis in Notch pathway activation by identifying a novel two-tier regulatory mechanism which may also be utilized by other chromatin remodeling factors.

Indexed as

Drosophila melanogasterDrosophila ProteinsHistonesReceptors, NotchSignal TransductionAnimalsHistone AcetyltransferasesRepressor ProteinsWings, AnimalDrosophila ProteinsHistone AcetyltransferasesHistonesN protein, DrosophilaReceptors, NotchRepressor ProteinsSu(H) protein, DrosophilaTip60 protein, DrosophilaDrosophilaH2AvNotchSu(H)Tip60

Identifiers

PMID40598498
PMCPMC12220669

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.