Evidence map›Paper›PMID 40598433›Full record

ArticleBMC biology2025

Adaptation of Diqing Tibetan pigs to hypoxic and cold environments through extramedullary hematopoiesis and uncoupled thermogenesis in the liver.

Xinpeng Li, Xinxing Dong, Shuhong Zhao, Yunlong Ma, Siqi Jin, Chunlu Zhou, Yi Lan, Shuyuan Luo, Bin Ni, Hongjiang Wei and 1 more

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In one paragraph

Article in BMC biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinpeng Li *Faculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Xinxing Dong *Faculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Shuhong ZhaoCollege of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Hubei, China.
Yunlong MaCollege of Animal Science and Technology, College of Animal Medicine, Huazhong Agricultural University, Hubei, China.
Siqi JinFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Chunlu ZhouFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Yi LanFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Shuyuan LuoFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Bin NiFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China.
Hongjiang WeiFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China. hongjiangwei@126.com.
Dawei YanFaculty of Animal Science and Technology, Yunnan Agricultural University, Yunnan, China. 1995027@ynau.edu.cn.

Funding

Major Science and Technology Special Projects of Yunnan Province 202202AE090005Young Talent Project of Yunnan Province Xing Dian Ying Talent Support Program XDYC-QNRC-2023-0394Yunnan Provincial Academician Expert Workstation Project 202305AF150128
6 · The paper itself

Abstract

backgroundTibetan pigs, which have long inhabited the Qinghai-Tibet Plateau at elevations exceeding 3000 m, serve as an ideal model for studying adaptation to extreme high-altitude environments. The multifunctionality of the liver (hematopoiesis, metabolism, detoxification) plays a crucial role in the adaptation of Tibetan pigs to hypoxic and cold environments, yet the specific mechanisms remain unclear.

resultsThis study employed single-nucleus RNA sequencing to profile and conduct bioinformatic analyses on the liver tissues of Diqing Tibetan pigs across 5 developmental stages (embryonic day 25, embryonic day 55, day of birth, development to 30 days, and development to 90 days). The goal was to identify and investigate key cell types and the regulatory mechanisms of core gene expression that facilitate adaptation to hypoxia and cold. In the fetal liver, various subpopulations of erythrocytes were detected. Analysis of these subpopulations allowed for the construction of a cellular development trajectory from proliferative erythrocytes to division erythrocytes to denucleating erythrocytes, revealing that key transcription factors (JUNB and MAFF) and functional genes (HBP1 and PPP2CB) play significant roles in erythrocyte enucleation. Furthermore, the presence of hematopoietic stem cells in the postnatal liver was observed, with identification of subpopulations exhibiting myeloid differentiation tendencies. These cells continuously support hematopoiesis and facilitate new blood vessel formation, thereby increasing red blood cell counts to aid in the adaptation of Diqing Tibetan pigs to hypoxic conditions. Additionally, a class of hepatocytes capable of converting metabolic energy into heat through uncoupling processes was identified, which assists in the adaptation to the cold environments of the plateau.

conclusionsIn summary, our study provides new scientific perspectives on the adaptation mechanisms of high-altitude animals to extreme environments.

Indexed as

AcclimatizationAdaptation, PhysiologicalCold TemperatureHematopoiesis, ExtramedullaryHypoxiaLiverThermogenesisAnimalsSwineTibetDiqing Tibetan pigHematopoietic stem cellsHepatocytesHypoxia and cryogenic adaptationSingle-nucleus transcriptome sequencing

Identifiers

PMID40598433
PMCPMC12218016

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.