ArticleParasites & vectors2025
New findings on the male reproductive system and spermatozoa of Aedes aegypti (Diptera: Culicidae).
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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5 authors.
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Abstract
backgroundAedes aegypti is one of the most important arbovirus vectors, characterized by its widespread distribution and exceptional reproductive capacity. This study reexamines the male reproductive system (MRS) of this species, focusing on its morphology throughout post-embryonic development and the structure of its spermatozoa.
methodsWe analyzed the MRS of A. aegypti in the larval L4, pupal, and adult stages using bright-field light microscopy, fluorescent microscopy, and transmission electron microscopy techniques. Spermatozoa measurements were made using the ImageJ software.
resultsIn L4 larvae, the MRS was composed of the two testes, a thin deferent duct, and a pair of seminal vesicles. The MRS is fully developed in pupae and adults, with two testes, deferent ducts, seminal vesicles, accessory glands, and an ejaculatory duct. Histological sections revealed that each testis is formed by a single follicle, which appeared to spiral at all stages. In pupae and adults, the testes showed germ cells at different stages of development, while the goblet portion of the deferent duct contained cytoplasmic bodies and spermatozoa. In adults, the seminal vesicles were filled with spermatozoa soon after emergence. Secretions from accessory glands were of the apocrine type. The spermatozoa were thin and long, measuring around 335 µm in length. Ultrastructural analysis revealed a very short acrosome covering the apical nucleus, in the flagellar region, an axoneme with the 9 + 9 + '1' microtubule pattern typical of mosquitoes, and two mitochondrial derivatives along the flagellum, narrowing at the terminal portion.
conclusionsOur analysis revealed a clear link between testicular development and spermatogenesis. In addition, we identified seminal vesicles at all life stages and accessory glands visible only in pupae and adults. The characterization of sperm structure and ultrastructure indicated similarities with other mosquito species. Finally, our study provided valuable information that may support research in comparative biology and reproduction.
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