Evidence map›Paper›PMID 40597583›Full record

ArticleBMC genomics2025

Unraveling key transposable elements in pathogen-induced bovine mastitis through comparative in vivo and in vitro transcriptomic analysis.

Songyan An, Siyuan Mi, Siqian Chen, Yongjie Tang, Yue Xing, Yi Jing, Ying Yu

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Songyan AnNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Siyuan MiNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Siqian ChenNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Yongjie TangNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Yue XingNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Yi JingBGI Research, Sanya, 572025, China.
Ying YuNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China. yuying@cau.edu.cn.

Funding

Earmarked Fund for Modern Agro-industry Technology Research System CARS-36National Key Research and Development Program of China 2023YFF1000902National Natural Science Foundation of China 32302706
6 · The paper itself

Abstract

backgroundBovine mastitis poses significant hazards to the yield and quality of dairy products, severely hindering the development of the dairy industry. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are well-established as two of the primary pathogens causing the disease. Transposable elements (TEs), occupying a notable proportion of livestock genomes, primarily function as regulatory elements modulating gene expression. Extensive studies have indicated that TEs contribute to transcriptional changes in the host during pathogen invasion. However, despite their potential significance, the key functional TEs associated with bovine mastitis remain unclear, highlighting the need to explore the critical roles of TEs in the immune processes of this disease.

resultsIn this study, in vitro and in vivo mastitis models were established using bovine mammary alveolar cells (Mac-T cells) and Chinese Holstein cows, respectively. In vitro findings showed distinct expression profiles of genes and TEs in response to challenges posed by S. aureus and E. coli. Specifically, 1,750 differentially expressed genes (DE Genes) and 3,046 differentially expressed TEs (DE TEs) were identified in the S. aureus challenge, while 2,353 DE Genes and 22,259 DE TEs were identified in the E. coli challenge. TEs were found to regulate the expression of genes primarily within immune-related pathways, including IL-17 and HIF-1 signaling pathways. TE-gene-QTL regulatory networks were established, providing preliminary insights into the molecular genetic mechanisms of TE regulation. By integrating in vitro and in vivo data, we identified and further validated two TE instances from MER53/DNA transposon and MIRc/SINE families as stably activated and repressed transcriptional markers for S. aureus mastitis, respectively.

conclusionsOur research underscores the potential regulatory roles of TEs in the pathogenesis of bovine mastitis and highlights their applicability as molecular markers for early diagnosis and prevention of this economically significant disease. Our study offers novel insights for the breeding and improvement of resistance to pathogen-induced mastitis in dairy cattle.

Indexed as

DNA Transposable ElementsGene Expression ProfilingMastitis, BovineStaphylococcal InfectionsTranscriptomeAnimalsCattleEscherichia coliEscherichia coli InfectionsFemaleStaphylococcus aureusDNA Transposable ElementsBovine mastitisEscherichia coliStaphylococcus aureusTranscriptomicsTransposable elements

Identifiers

PMID40597583
PMCPMC12210757

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.