ArticleCancer imaging : the official publication of the International Cancer Imaging Society2025
Fluorescence imaging of MALAT1 expression using a Cy5.5-labeled antisense oligonucleotide in lung cancer and epidermal carcinoma cells.
Article in Cancer imaging : the official publication of the International Cancer Imaging Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Nanocarriers engineering for efficient NSUN2 silencing and immune responsive therapy in metastatic colorectal cancer.Materials today. Bio · 2026Article
- Engineering Anti-MMP-9 Peptide-Modified Nanoparticles for Precision MRI Diagnosis of Endometriosis.Molecular imaging and biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundThe long noncoding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) has been extensively studied as an oncogenic factor. Antisense oligonucleotides (ASOs) labeled with the Cyanine5.5 (Cy5.5) dye enable effective in vivo imaging using near-infrared fluorescence.
methodsPan-cancer research on MALAT1 expression levels was conducted through The Cancer Genome Atlas (TCGA) database analysis. The selectivity and specificity of MALAT1-ASO were validated in lung cancer and epidermal carcinoma cell lines (A549, A431, PC9GR, and PC9) using cellular fluorescence and flow cytometry. Corresponding xenograft models were created for these cell lines, and near-infrared fluorescence imaging assessed tumor imaging effectiveness and the biodistribution of Cy5.5-labeled MALAT1 ASOs.
resultsMALAT1 expression levels were found to be upregulated in various tumors and high MALAT1 expression level correlated to poor prognosis in some tumors. The high expression of MALAT1 was confirmed in tumor cell lines. In vitro fluorescent intensity correlated with MALAT1 expression within cells. The fluorescence intensity also exhibited concentration dependence. In vivo experiments revealed a significant contrast between tumor tissues and normal tissues within 24 h. Tumors exhibited varied probe uptake corresponding to their MALAT1 expression levels. Ex vivo experiments shows high probe uptake in kidney, liver and intestine tissues.
conclusionMALAT1 is highly expressed in various cancer tissues and associated with poor prognosis. In xenograft models of lung cancer and epidermal carcinoma cell lines A549, A431, PC9GR, and PC9, Cy5.5-labeled ASOs exhibit evident binding specificity and discernible imaging effect in both in vitro and in vivo, effectively reflecting MALAT1 expression levels in tumors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.