Evidence map›Paper›PMID 40597390›Full record

ArticleStem cell research & therapy2025

The circ_0054633/miR-590-3p/RUNX2 positive feedback loop promotes the osteogenic differentiation of BMSCs.

Lingxiao Wang, Yang Liu, Zhanqiu Diao, Yishu Huang, Haoqing Yang, Yue Zhang, Bowen Zhou, Zhenhua Gao, Zhaochen Shan, Jun Li and 1 more

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In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lingxiao WangLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Yang LiuLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Zhanqiu DiaoLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Yishu HuangLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Haoqing YangLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Yue ZhangDepartment of Periodontics, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Bowen ZhouOutpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Zhenhua GaoDepartment of Dental Implant Center, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Zhaochen ShanOutpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Jun LiDepartment of Dental Implant Center, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
Zhipeng FanLaboratory of Molecular Signalling and Stem Cell Therapy, Beijing Key Laboratory for Tooth Regeneration and Function Reconstruction of Oral Tissues, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China. zpfan@ccmu.edu.cn.ORCID http://orcid.org/0000-0003-1662-9420

Funding

National Natural Science Foundation of China 82401155
6 · The paper itself

Abstract

backgroundMandibular bone marrow stem cells (BMSCs) from patients with type 2 diabetes mellitus (T2DM) have poor osteogenic differentiation capacity. Elucidating the molecular mechanisms by which circular RNAs (circRNAs) play specific roles in T2DM will reveal new diagnostic biomarkers and therapeutic targets.

methodsBMSCs with different circ_0054633 expression levels were generated. Furthermore, alkaline phosphatase (ALP) activity, alizarin red staining (ARS), and transplantation of HA/tricalcium phosphate into BMSCs were performed to detect the osteogenic effects of different levels of circ_0054633 expression in BMSCs in vivo and in vitro.

resultsIn this study, we identified 80 differentially expressed circRNAs in jawbone-derived BMSCs from patients with T2DM. Notably, significant downregulation of circ_0054633 promoted the osteogenic differentiation of these cells in vitro and in vivo. Mechanistically, circ_0054633 acts as a miRNA sponge; specifically, it actively regulates the expression of RUNX2 by sponging miR-590-3p and thus promoting the osteogenic differentiation of the BMSCs. In addition, we found that circ_0054633 was a direct transcriptional target of RUNX2. RUNX2 overexpression activated the circ_0054633 promoter and promoted the generation of nuclear circ_0054633, whereas RUNX2 knockdown abrogated the osteogenic role of circ_0054633 and formed a circ_005463/miR-590-3p/RUNX2 positive feedback loop.

conclusionsOur results suggest that the circ_0054633/miR-590-3p/RUNX2 positive feedback loop promotes the osteogenic differentiation of BMSCs and is expected to be a potential biomarker and therapeutic target for bone regeneration in T2DM.

Indexed as

Cell DifferentiationCore Binding Factor Alpha 1 SubunitMesenchymal Stem CellsMicroRNAsOsteogenesisRNA, CircularAnimalsCells, CulturedDiabetes Mellitus, Type 2HumansMaleMiceCore Binding Factor Alpha 1 SubunitMicroRNAsMIRN590 microRNA, humanRNA, CircularRUNX2 protein, humanBMSCscirc_0054633miR-590-3pOsteogenic differentiationRUNX2

Identifiers

PMID40597390
PMCPMC12210457

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.