Evidence map›Paper›PMID 40596834›Full record

ArticleBMC plant biology2025

Genome-wide identification and functional analysis of R2R3-MYB genes in Acer pseudosieboldianum: insights into low temperature stress response and anthocyanin biosynthesis.

Shikai Zhang, Dong Zeng, Heng Zhang, Luwei Ding, Dongyuan Guo, Peilin Sun, Zhiming Han, Guanzheng Qu, Tuya Siqin, Xiangling You

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In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Shikai Zhang *Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Dong Zeng *State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Heng ZhangState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Luwei DingState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Dongyuan GuoState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Peilin SunHeilongjiang Institute of Atomic Energy, Harbin, 150081, China.
Zhiming HanState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Guanzheng QuState Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China.
Tuya SiqinHeilongjiang Institute of Atomic Energy, Harbin, 150081, China. siqintuya-80@outlook.com.
Xiangling YouKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China. youxiangling@nefu.edu.cn.

Funding

External Cooperation Program of Heilongjiang Academy of Sciences DWHZ2023YZNY01Fundamental Research Funds for the Central Universities 2572023AW43The Opening Project of State Key Laboratory of Tree Genetics and Breeding K2021102
6 · The paper itself

Abstract

The genus Acer pseudosieboldianum, a cold-tolerant maple species native to northeastern China, exhibits remarkable adaptability to extreme winters and vibrant autumn foliage, yet the molecular mechanisms underlying these traits remain poorly characterized. Here, we conducted the first genome-wide identification and functional analysis of the R2R3-MYB transcription factor family in A. pseudosieboldianum to elucidate its roles in cold stress response and anthocyanin biosynthesis. Using a combination of bioinformatic approaches and transcriptomic profiling, we identified 95 ApseMYB genes, classified into 12 subfamilies with conserved motif architectures and structural features. Cis-regulatory element analysis identified 34 genes enriched in low-temperature response motifs, while transcriptomic and qPCR validation highlighted 22 cold-responsive and 14 leaf color-associated ApseMYB genes, including ApseMYB31 (28-fold induction at 4 °C) and ApseMYB16 (52-fold upregulation during leaf reddening). Subcellular localization analysis indicated that ApseMYB31 and ApseMYB16 predominantly localize to the nucleus, consistent with the canonical nuclear activity of R2R3-MYB transcription factors. This study revealed that members of the R2R3-MYB gene family in A. pseudosieboldianum play pivotal roles in mediating both low temperature resistance and leaf anthocyanin accumulation, providing new insights for molecular breeding of cold-tolerant ornamental trees.

Indexed as

AcerAnthocyaninsCold-Shock ResponsePlant ProteinsTranscription FactorsCold TemperatureGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGenome, PlantPhylogenyAnthocyaninsPlant ProteinsTranscription FactorsAcer pseudosieboldianumAnthocyanin biosynthesisLow temperature stressR2R3-MYB gene family

Identifiers

PMID40596834
PMCPMC12219217

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.