ArticleScientific reports2025
In vitro activity of phages against periprosthetic joint infection-associated staphylococcal biofilms.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bacteriophages in Hip and Knee Periprosthetic Joint Infections: A Promising Tool in the Era of Antibiotic Resistance.Medical sciences (Basel, Switzerland) · 2025Pooled it
- Isolation and characterization of a novelFrontiers in cellular and infection microbiology · 2026Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lytic phages are potential therapeutic options, based on their ability to lyse bacteria in vitro. Although many infection-types for which phage therapy is being considered involve biofilms, in vitro anti-biofilm activity of phage is poorly defined, in part due to a lack of standardized methods for assessment. Here, phages SaMD07phi1 and SaRBI05030phi5 were evaluated against Staphylococcus aureus SaMD07 and SaRBI05030, respectively, in biofilms formed in 96-well plates and on glass beads, and planktonically, in TSB and PBS, with endpoints including by CFUs and Biolog Omnilog hold times. The bead biofilm assay in TSB using the Omnilog (BBTO) was employed to test eight staphylococcal phages against S. aureus, Staphylococcus epidermidis, and Staphylococcus lugdunensis from periprosthetic joint infection. Biofilms on beads in TSB showed better eradication than in microtiter wells, with no significant changes with PBS in either format. CFU counts and Omnilog units correlated linearly through 8 h of testing. In the bead assay, CFU counts showed that phage SaMD07phi1 eliminated growth at 4 h, while SaRBI05030phi5 achieved a ~ 3-log reduction at 8 h; with Omnilog hold times of 37 and 28 h, respectively. Diverse activity and good reproducibility of the BBTO was observed among 8 phages, with SaMD07phi1 showing the highest activity. In conclusion the BBTO is a promising potential method for biofilm susceptibility testing.
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