Evidence map›Paper›PMID 40596474›Full record

ArticleCommunications biology2025

Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.

Kaela M Varberg, Ayelen Moreno-Irusta, Allynson Novoa, Brynne Musser, Joseph M Varberg, Jeremy P Goering, Irfan Saadi, Khursheed Iqbal, Hiroaki Okae, Takahiro Arima and 3 more

Abstract read
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Modeling Aspects of Human Placental Development with Trophoblast Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Kaela M Varberg *Institute for Reproductive and Developmental Sciences, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA.
Ayelen Moreno-Irusta *Institute for Reproductive and Developmental Sciences, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA.ORCID http://orcid.org/0000-0001-6810-6475
Allynson NovoaDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Brynne MusserInstitute for Reproductive and Developmental Sciences, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA.
Joseph M VarbergStowers Institute for Medical Research, Kansas City, MO, USA.ORCID http://orcid.org/0000-0002-5235-5896
Jeremy P GoeringDepartment of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
Irfan SaadiDepartment of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, KS, USA.ORCID http://orcid.org/0000-0002-6250-6651
Khursheed IqbalInstitute for Reproductive and Developmental Sciences, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA.
Hiroaki OkaeDepartment of Trophoblast Research, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Takahiro ArimaDepartment of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Sendai, Japan.
John WilliamsDepartment of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Margareta D PisarskaDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. margareta.pisarska@cshs.org.ORCID http://orcid.org/0000-0003-1096-6506
Michael J SoaresInstitute for Reproductive and Developmental Sciences, Department of Pathology & Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA. msoares@kumc.edu.ORCID http://orcid.org/0000-0001-7158-1592

Funding

TROPHOBLAST DIFFERENTIATIONR01HD020676 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MICHAEL J SOARES · 1986 to 2026
$10.1M
Anti-Coagulation Factors and PlacentationR01HD099638 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI SOARES, MICHAEL J · 2019 to 2023
$2.5M
The impact of sex and gender on disease progression, from developmental originsR01AI154535 · NIAID · CEDARS-SINAI MEDICAL CENTER · PI PISARSKA, MARGARETA · 2020 to 2024
$2.3M
Trophoblast-Guided Uterine Transformation in the Establishment of PregnancyR01HD105734 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MICHAEL J SOARES · 2022 to 2026
$2.2M
Pathways Affecting Trophoblast DevelopmentR01HD112559 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MICHAEL J SOARES, Geetu Tuteja · 2024 to 2026
$1.9M
Noncoding RNA regulation of the human placental transcriptome among the sexesR01HD091773 · NICHD · CEDARS-SINAI MEDICAL CENTER · PI PISARSKA, MARGARETA · 2017 to 2018
$900k
Early Events in Extravillous Trophoblast Cell DifferentiationK99HD115834 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MORENO, AYELEN · 2024 to 2025
$261k
Supplement (Covid) to Regulation of endovascular trophoblast cell development and uterine spiral artery remodelingF32HD096809 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI VARBERG, KAELA MARGARET · 2018 to 2021
$232k
Regulation of Invasive Trophoblast Cell Lineage DevelopmentK99HD107262 · NICHD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI VARBERG, KAELA MARGARET · 2022 to 2023
$142k
NIAID NIH HHS R01 AI154535NICHD NIH HHS F32 HD096809NICHD NIH HHS K99 HD107262NICHD NIH HHS K99 HD115834NICHD NIH HHS R01 HD020676NICHD NIH HHS R01 HD091773NICHD NIH HHS R01 HD099638NICHD NIH HHS R01 HD105734NICHD NIH HHS R01 HD112559U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) F32HD096809U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) HD020676U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) HD099638U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) HD105734U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) K99HD107262U.S. Department of Health & Human Services | NIH | Office of Research on Women's Health (NIH Office of Research on Women's Health) AI154535
6 · The paper itself

Abstract

Human trophoblast stem (TS) cells are informative in vitro models for the generation and testing of biologically meaningful hypotheses. The goal of this project was to derive patient-specific TS cell lines from clinically available chorionic villus biopsies. Cell outgrowths were captured from human chorionic villus tissue specimens cultured in modified human TS cell medium. Cell colonies emerged early during the culture and cell lines were established and passaged for several generations. Karyotypes of the newly established chorionic villus-derived trophoblast stem (TS

Indexed as

Chorionic VilliStem CellsTrophoblastsBiopsyCell DifferentiationCell LineChorionic Villi SamplingFemaleHumansPregnancy

Identifiers

PMID40596474
PMCPMC12215853

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.