Evidence map›Paper›PMID 40596169›Full record

ArticleScientific reports2025

Antigenicity analysis of the recombinant outer membrane NlpI protein of Mannheimia haemolytica.

Xu Shuyun, Sun Yingni, Wei Jingjing, Wei Zengke, Yu Jie, Jiang Meiqi, Luo Chaofan, Zhang Hui, Wang Zhen, Wang Zijie and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xu ShuyunCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Sun YingniSydney School of Veterinary Science, The University of Sydney, Camperdown, Australia.
Wei JingjingCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Wei ZengkeCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Yu JieCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Jiang MeiqiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Luo ChaofanDepartment of Prosthodontics, The First Affiliated Hospital/Affiliated Stomatological Hospital of Xinjiang Medical University, Urumqi, 830054, China.
Zhang HuiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Wang ZhenCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Wang ZijieCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Wu JieXinjiang Production and Construction Corps Eighth Division Animal Husbandry and Aquatic Development Service Center, Shihezi, 832000, Xinjiang, China. 18997888015@163.com.
Zhou XiaCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. zhoux993@163.com.

Funding

Corps eighth division key areas of science and technology research projects 2023NY03-1Corps key areas of science and technology research 2021AB012Corps key areas of science and technology research 2024AB035
6 · The paper itself

Abstract

To explore the antigenic characteristics of the NlpI protein. This study screened a potential antigen of Mannheimia haemolytica using NCBI Blast analysis and investigated its immunogenicity using online software. The recombinant NlpI protein (rNlpI) was expressed in prokaryotic cells, and its reactogenicity was detected by western blot. The transcription level of nlpI mRNA in M. haemolytica after various culture durations was determined by RT-qPCR. Mice were challenged with M. haemolytica strain 95 at an LD

Indexed as

Antigens, BacterialBacterial Outer Membrane ProteinsMannheimia haemolyticaAnimalsAntibodies, BacterialBacterial VaccinesFemaleImmunizationMiceMice, Inbred BALB CPasteurellaceae InfectionsRecombinant ProteinsAntibodies, BacterialAntigens, BacterialBacterial Outer Membrane ProteinsBacterial VaccinesRecombinant ProteinsAntigenicityBioinformatics characteristicMannheimia haemolyticaNlpI proteinProkaryotic expressionTranscription level

Identifiers

PMID40596169
PMCPMC12218578

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.