Evidence map›Paper›PMID 40595888›Full record

ArticleScientific reports2025

Enrichment of ornithine decarboxylase degron transduced colorectal cancer cells for extended application of cancer stem cell models.

Ryo Ikeshima, Hidekazu Takahashi, Hiroyuki Yamamoto, Shihori Kouda, Rika Akisue, Manami Tsujimoto, Yuhki Yokoyama, Haruka Hirose, Hiroaki Itakura, Yoshihiro Morimoto and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Ryo IkeshimaDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Hidekazu TakahashiDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Hiroyuki YamamotoDepartment of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Yamadaoka 1-7, Suita, Osaka, 565-0871, Japan.
Shihori KoudaDepartment of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Yamadaoka 1-7, Suita, Osaka, 565-0871, Japan.
Rika AkisueDepartment of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Yamadaoka 1-7, Suita, Osaka, 565-0871, Japan.
Manami TsujimotoDepartment of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Yamadaoka 1-7, Suita, Osaka, 565-0871, Japan.
Yuhki YokoyamaDepartment of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Yamadaoka 1-7, Suita, Osaka, 565-0871, Japan.
Haruka HiroseDepartment of Computational and Systems Biology, Medical Research Institute, Institute of Science Tokyo, Tokyo, 113-0034, Japan.
Hiroaki ItakuraDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Yoshihiro MorimotoDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Norikatsu MiyoshiDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Mamoru UemuraDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Daisuke OkuzakiGenome Information Research Centre, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Shinji TanakaDepartment of Molecular Oncology, Graduate School of Medicine, Institute of Science Tokyo, Tokyo, 113-8510, Japan.
Hidetoshi EguchiDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Yuichiro DokiDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan.
Hirofumi YamamotoDepartment of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, 565-0871, Japan. hyamamoto@sahs.med.osaka-u.ac.jp.
Masaki MoriGraduate School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer stem cells (CSCs) are present in small quantities in tumor populations. To permit various analyses of CSCs, we attempted to enrich and expand ornithine decarboxylase (ODC) degron-transduced colorectal cancer (CRC) cells, which retain low proteasome activity. ZsGreen fluorescence-positive (ZsGreen

Indexed as

Colorectal NeoplasmsNeoplastic Stem CellsOrnithine DecarboxylaseAnimalsCell Line, TumorDegronsDrug Resistance, NeoplasmFluorouracilGene Expression Regulation, NeoplasticHCT116 CellsHumansMiceMicroRNAsOxaliplatinTransduction, GeneticFluorouracilMicroRNAsOrnithine DecarboxylaseOxaliplatin

Identifiers

PMID40595888
PMCPMC12216209

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.