Evidence map›Paper›PMID 40595566›Full record

ArticleNature communications2025

Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans.

Thiago L Knittel, Brooke E Montgomery, Kailee J Reed, Madeleine C Chong, Ida J Isolehto, Erin R Cafferty, Margaret J Smith, Reese A Sprister, Colin N Magelky, Hataichanok Scherman and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thiago L Knittel *Department of Biology, Colorado State University, Fort Collins, CO, USA.ORCID http://orcid.org/0000-0001-5716-2033
Brooke E Montgomery *Department of Biology, Colorado State University, Fort Collins, CO, USA.
Kailee J ReedDepartment of Biology, Colorado State University, Fort Collins, CO, USA.
Madeleine C ChongDepartment of Biology, Colorado State University, Fort Collins, CO, USA.ORCID http://orcid.org/0009-0009-9683-4176
Ida J IsolehtoBiology of Non-coding RNA group, Institute of Molecular Biology, Mainz, Germany.
Erin R CaffertyDepartment of Biology, Colorado State University, Fort Collins, CO, USA.ORCID http://orcid.org/0000-0001-8771-5241
Margaret J SmithDepartment of Biology, Colorado State University, Fort Collins, CO, USA.
Reese A SpristerDepartment of Biology, Colorado State University, Fort Collins, CO, USA.
Colin N MagelkyDepartment of Biology, Colorado State University, Fort Collins, CO, USA.
Hataichanok SchermanDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.
René F KettingBiology of Non-coding RNA group, Institute of Molecular Biology, Mainz, Germany.ORCID http://orcid.org/0000-0001-6161-5621
Taiowa A MontgomeryDepartment of Biology, Colorado State University, Fort Collins, CO, USA. tai.montgomery@colostate.edu.ORCID http://orcid.org/0000-0001-7857-3253

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Mechanism and function of small RNAs in development and gene silencing in the germlineR35GM119775 · NIGMS · COLORADO STATE UNIVERSITY · PI Taiowa Akoni Montgomery · 2016 to 2026
$4.3M
Deutsche Forschungsgemeinschaft (German Research Foundation) 252386272Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) R35GM119775NIGMS NIH HHS R35 GM119775NIH HHS P40 OD010440
6 · The paper itself

Abstract

Primary microRNA (pri-miRNA) transcripts are processed by the Microprocessor, containing the ribonuclease Drosha and its RNA-binding partner DGCR8/Pasha. In a forward genetic screen utilizing a fluorescence-based sensor that monitors pri-miRNA processing in live Caenorhabditis elegans, we identify a mutation in the conserved G179 residue adjacent to the namesake W180 of Pasha's WW domain that disrupts pri-miRNA processing. We show that both the G179 and W180 residues are required for Pasha dimerization and Microprocessor assembly. The WW domain also facilitates nuclear localization of Pasha, likely through its role in Microprocessor assembly, which in turn promotes nuclear enrichment of Drosha. Furthermore, depletion of Pasha mislocalizes Drosha to the cytoplasm, and vice versa, while deletion of Pasha's N-terminus causes both proteins to accumulate in nucleoli. Our results reveal a mutual dependency between Pasha and Drosha for their localization in C. elegans and highlight the role of Pasha's WW domain in maintaining Microprocessor integrity.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsRibonuclease IIIRNA-Binding ProteinsAnimalsCell NucleusMicroRNAsMutationRNA Processing, Post-TranscriptionalCaenorhabditis elegans ProteinsMicroRNAsRibonuclease IIIRNA-Binding Proteins

Identifiers

PMID40595566
PMCPMC12219410

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.