Evidence map›Paper›PMID 40595388›Full record

ArticleScientific reports2025

Liver developmental microenvironment promotes iHSC generation from human iPSCs.

Di Ye, Min Ding, Yu-Mu Song, Heng-Xing Meng, Wen-Hao Chen, Jian-Yun Ge, Yun-Wen Zheng

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Di Ye *Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China.
Min Ding *Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China.
Yu-Mu SongGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China.
Heng-Xing MengHaihe Laboratory of Cell Ecosystem, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin, 300020, China.
Wen-Hao ChenGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China.
Jian-Yun GeGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China. gejianyun85@gmail.com.
Yun-Wen ZhengGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and South China, Institute of Large Animal Models for Biomedicine, School of Pharmacy and FoodEngineering, Wuyi University, Jiangmen, 529020, Guangdong, China. zhengyunwen@ihcams.ac.cn.

Funding

China Foundation For Youth Entrepreneurship and Employment -Incaier Public Welfare Fund HH25KYHX0003Haihe Laboratory of Cell Ecosystem Innovation Fund HH24KYZX0008National Natural Science Foundation of China 82270697the Guangdong Basic and Applied Basic Research Foundation 2023A1515012574the Jiangsu Provincial Medical Key Discipline Cultivation Unit JSDW202229the Science and Technology Planning Project of Guangdong Province of China 2021B1212040016
6 · The paper itself

Abstract

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells that play a crucial role in liver formation and regeneration, as well as in different pathological diseases. However, the limited source of primary HSCs (pHSCs) and the suboptimal functionality of induced HSCs (iHSCs) by existing methods restrict their application in biomedical modeling. We developed a de novo differentiation method to generate iHSCs under simulated liver microenvironment in vitro, thereby enhancing the function of the differentiated cells. These iHSCs exhibited key HSC functions, including the expression of α-smooth muscle actin, collagen, and the capability to store Vitamin A. RNA sequencing further revealed that the present iHSCs converged more closely to pHSCs with very similar transcriptional profile compared to the established conventional induction. Additionally, the novel HSC-specific marker genes, FBLN5, NID2, and SVEP1 were identified by RNA sequencing and gene expression assay. In conclusion, our novel differentiation approach enables the generation of iHSCs with phenotypic and functional traits similar to those of pHSCs. The generation of highly functional iHSCs may make it more feasible to accurately simulate the liver-specific multicellular microenvironments, thus providing new perspectives on the modeling of physiological regenerative processes and disease progression in the liver, as well as useful tools for creating of new therapeutic strategies.

Indexed as

Cellular MicroenvironmentHepatic Stellate CellsInduced Pluripotent Stem CellsLiverCell DifferentiationCells, CulturedHumans

Identifiers

PMID40595388
PMCPMC12214545

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