Evidence map›Paper›PMID 40595174›Full record

ArticleScientific reports2025

A new bacteriolytic amidase Ami of Lysobacter capsici XL1.

Irina Kudryakova, Alexey Afoshin, Sergey Tarlachkov, Sofya Pavlenko, Natalia Suzina, Nina Shishkova, Elena Leontyevskaya, Natalia Leontyevskaya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Genomic Features of the MicropredatorInternational journal of molecular sciences · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Irina KudryakovaLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Alexey AfoshinLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Sergey TarlachkovLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Sofya PavlenkoLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Natalia SuzinaLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Nina ShishkovaFBIS State Research Center for Applied Biotechnology and Microbiology, 24 Kvartal-A Territory, Obolensk, Russia, 142279.
Elena LeontyevskayaLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290.
Natalia LeontyevskayaLaboratory of Microbial Cell Surface Biochemistry, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC PSCBR, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region, Russia, 142290. vasilyevanv@rambler.ru.

Funding

Russian Science Foundation 24-75-10078
6 · The paper itself

Abstract

One of the most pressing issues in modern biomedicine is the search for new antimicrobial agents - antibiotics, peptides, bacteriolytic enzymes. This study, using transcriptomic and proteomic approaches, identified a new extracellular bacteriolytic enzyme of Lysobacter capsici XL1 - the amidase Ami. The enzyme was isolated and characterized. Ami was found to hydrolyze the amide bond between the carbohydrate and peptide fragments in bacterial peptidoglycans of chemotypes A1γ, A3α, and A4α. Ami lysed live target cells of opportunistic bacteria Micrococcus luteus Ac-2230

Indexed as

AmidohydrolasesBacterial ProteinsLysobacterAnti-Bacterial AgentsBacillus cereusPeptidoglycanProteomicsamidaseAmidohydrolasesAnti-Bacterial AgentsBacterial ProteinsPeptidoglycanBacterial bacteriolytic enzymeHomologous expression systemLysobacterNew bacteriolytic amidaseOmicsPeptidoglycan types

Identifiers

PMID40595174
PMCPMC12219114

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.