ArticleScientific reports2025
Study of caspase-6 activity in aggressive HCT116 cells using methotrexate-encapsulated lactoferrin-conjugated solid lipid nanoparticles via in silico and in vitro approaches.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Modeling Microbiome Modulation of Tumor Metabolic Networks to Predict Synergistic Therapies.bioRxiv : the preprint server for biology · 2026Article
- Neural regulatory networks: challenges and opportunities in the treatment of psoriasis.Frontiers in immunology · 2026Review
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4 authors.
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Abstract
Methotrexate-encapsulated solid lipid nanoparticles (MTX-SLNs) and lactoferrin-decorated MTX-loaded nanoparticles (MTX-Lf-SLNs) present a promising strategy for treating colorectal cancer. Among different molecular targets, MTX demonstrated the highest affinity for Caspase-6, exhibiting a docking score of -9.316, while molecular dynamics validated stable interactions. The optimized nanoparticles displayed a spherical shape (~ 160 nm, as observed in TEM images) with a high drug encapsulation efficiency of 85.87% for MTX-SLNs and 80.11% for MTX-Lf-SLNs, which ensured improved stability. Structural analyses using FTIR, DSC confirmed effective drug encapsulation and the binding of lactoferrin. Interestingly, MTX-Lf-SLNs demonstrated higher cytotoxicity (IC50: 0.51 µM) compared to MTX-SLNs and free MTX, inducing apoptosis and stopping cell cycle progression in HCT116 cells. This improved effect was associated with receptor-driven absorption through lactoferrin targeting. Nanoparticulate formulations decreased TNF-α (17.6 ± 2.1 pg/mL), IL-6 (20.2 ± 1.9 pg/mL), and IL-1β (15.4 ± 3.4 pg/mL), thereby reducing immune activation. The nanoparticles exhibited extended, pH-sensitive drug release (70% at pH 5.7) and significant anti-angiogenic effects (~ 70% inhibition in CAM assay). Moreover, they enhanced the balance of reactive oxygen species and safeguarded mitochondria, thereby lowering overall toxicity. Migration assays further validated their capacity to obstruct cancer cell invasiveness, suggesting a potential to impede metastasis. Utilizing the bioactivity of lactoferrin for precise delivery, MTX-Lf-SLNs offer an attractive approach to enhance anti colon cancer efficacy while reducing unwanted side effects.
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