Evidence map›Paper›PMID 40594216›Full record

ArticleScientific reports2025

Optimizing Aedes albopictus rearing: effects of insect- and bacteria-based larval diets on immature and adult performance.

Eleni C Savvidou, Georgios A Kyritsis, Evangelia D Mpakovasili, Stefania P Kaltsou, Venetia Ch Karathanasi, Antonios A Augustinos, George Tsiamis, Christos I Rumbos, Christos G Athanassiou, Antonios Michaelakis and 1 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Eleni C SavvidouDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece.
Georgios A KyritsisDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece.
Evangelia D MpakovasiliDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece.
Stefania P KaltsouDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece.
Venetia Ch KarathanasiDepartment of Plant Protection Patras, Institute of Industrial and Forage Crops, Hellenic Agricultural Organization 'DIMITRA', Patras, 26442, Greece.
Antonios A AugustinosDepartment of Plant Protection Patras, Institute of Industrial and Forage Crops, Hellenic Agricultural Organization 'DIMITRA', Patras, 26442, Greece.
George TsiamisLaboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, Agrinio, 30131, Greece.
Christos I RumbosDepartment of Agriculture, School of Agricultural Sciences, University of Patras, Messolonghi, 30200, Greece.
Christos G AthanassiouDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece.
Antonios MichaelakisLaboratory of Insects & Parasites of Medical Importance, Scientific Directorate of Entomology and Agricultural Zoology, Benaki Phytopathological Institute, Kifissia Athens, 14561, Greece. a.michaelakis@bpi.gr.
Nikos T PapadopoulosDepartment of Agriculture, Crop Production and Rural Environment Laboratory of Entomology and Agricultural Zoology, University of Thessaly, Magnisias, 38446, Greece. nikopap@uth.gr.

Funding

MoSquITo project Greece 2.0; EU; T2EDK-02020
6 · The paper itself

Abstract

Mass production of Aedes albopictus for Sterile Insect Technique (SIT) requires cost-effective and nutritionally balanced larval diets to ensure high survival, optimal development and competitive adult fitness. This study evaluates the potential of insect-derived meals and dead autoclaved bacteria as the main protein sources in mosquito larval diets. Four isoproteinic diets were studied, each one incorporating different protein sources: Brewer's yeast (CAA), Tenebrio molitor meal (UTH-YM), Hermetia illucens meal (UTH-BSF) and Enterobacter spp. dry biomass (UTH-ENT). Results demonstrated immature survival higher than 78% for all diets. Developmental duration varied significantly across diets. The UTH-ENT diet extended larval development and conferred greater wing length. The highest protandry percentage was observed in the CAA diet, facilitating sex separation for SIT implementation. Adult survival over 20 days varied significantly among diets but not sexes. Males from the UTH-BSF diet exhibited the highest survival rate, while females from UTH-ENT diet showed the shortest lifespan. Given the rising costs and the variability in the quality of brewer's yeast, our findings support the integration of insect-based diets as sustainable protein source alternatives for Ae. albopictus mass-rearing. Further research should refine larval diet formulations considering that combining different protein sources may enhance rearing success, to facilitate SIT efficiency and sustainable mosquito suppression.

Indexed as

AedesAnimal FeedDietAnimalsFemaleLarvaMaleEnterobacter spp.Insect mealsIsoproteinic dietsMosquitoesSterile insect technique (SIT)

Identifiers

PMID40594216
PMCPMC12216696

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.