Evidence map›Paper›PMID 40593901›Full record

ArticleScientific reports2025

Evaluating the InSignia IFI27 expression assay for detecting viral respiratory infection compared to a traditional gene normalisation assay.

Tiana M Pelaia, Karan Kim, Nicole Lima, Claire Gibbs, Lee M Smith, Sally Teoh, Ya Wang, Colin Denver, Elisa Mokany, Alison Todd and 4 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Tiana M Pelaia *Department of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia. tpel0110@uni.sydney.edu.au.
Karan Kim *Centre for Immunology and Allergy Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, Australia.
Nicole LimaSpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Claire GibbsSpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Lee M SmithSpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Sally TeohDepartment of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia.
Ya WangDepartment of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia.
Colin DenverSpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Elisa MokanySpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Alison ToddSpeeDx Pty Ltd, Eveleigh, NSW, Australia.
Sam OrdeDepartment of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia.
Benjamin TangCentre for Immunology and Allergy Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, Australia.
Anthony McLeanDepartment of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia.
Maryam ShojaeiDepartment of Intensive Care Medicine, Nepean Hospital, Penrith, NSW, Australia. maryam.shojaei@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Host gene expression is crucial for understanding disease progression and developing diagnostic biomarkers. Previously, we identified a novel immune biomarker IFI27, validated with routine RT-qPCR methods employed in a research setting, that discriminates between influenza and bacteria in patients with suspected respiratory infection. This study aimed to assess the InSignia method, which employs a novel gene normalization technique to yield a variable transcript analysis (VITA) index. The VITA index measures gene expression relative to a non-transcribed region of DNA, such that it is independent of sample quality or quantity. We compared IFI27 gene expression measured by the InSignia assay to that of the research assay in blood samples collected from patients with respiratory diseases and SARS-CoV-2 vaccinated individuals. The study found a strong correlation and acceptable agreement between traditional ΔCq methods and InSignia for IFI27 levels in the higher range (log(ΔCq)

Indexed as

COVID-19Membrane ProteinsRespiratory Tract InfectionsBiomarkersFemaleGene Expression ProfilingHumansMaleMiddle AgedSARS-CoV-2BiomarkersIFI27 protein, humanMembrane Proteins

Identifiers

PMID40593901
PMCPMC12216230

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.