Evidence map›Paper›PMID 40593769›Full record

ArticleScientific reports2025

Non-invasive assessment of stem cell DNA integrity in response to environmental stressors.

Juan Wang, Yue Zhou, Lifan Zhuo, Bei Yan, Lianghong Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Juan Wang *Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Human Sperm Bank, Institute of Medical Sciences, Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, 750004, China.
Yue Zhou *Department of Urology, General Hospital of Ningxia Medical University, Yinchuan, 750001, China.
Lifan Zhuo *Guizhou Provincial Center for Disease Control and Prevention, Guiyang, 550000, China.
Bei YanKey Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Human Sperm Bank, Institute of Medical Sciences, Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, 750004, China. d202081630@alumni.hust.edu.cn.
Lianghong MaKey Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Human Sperm Bank, Institute of Medical Sciences, Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, 750004, China. mmm1770@163.com.

Funding

China Postdoctoral Science Foundation General Funding Project 2023MD744222National Natural Science Foundation of China 82260290Natural Science Foundation of Ningxia Province 2023AAC03659The Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education scientific research project XZ2024013The Key Project of Research and Development of Ningxia Hui Autonomous Region of China 2020BFH02002
6 · The paper itself

Abstract

The maintenance of DNA integrity in spermatogonial stem cells (SSCs) is essential for ensuring genetic stability and proper function in male germline cells. Here, we established a non-invasive approach to evaluate SSC DNA integrity and investigated the impact of detrimental environmental stressors on genomic stability. SSCs were isolated and cultured from mouse testes. SSCs identity was confirmed through immunofluorescence staining and flow cytometric analysis of specific molecular markers.DNA strand breaks were detected using a novel TdT enzyme-Endo IV-fluorescent probe biosensor, confirming the methodological feasibility. Heat stress and cryopreservation models were established, with the biosensor quantifying stress-induced DNA damage in SSCs. Additionally, extracellular free DNA fragments in culture supernatants were quantified as a non-invasive measure of SSCs DNA integrity.

Indexed as

DNADNA DamageSpermatogoniaStem CellsStress, PhysiologicalAnimalsCells, CulturedCryopreservationGenomic InstabilityHeat-Shock ResponseMaleMiceMice, Inbred C57BLTestisDNAHeat stressMean number of DNA breakpointNon-invasive detectionProbe biosensorReactive oxygen speciesSpermatogonial stem cells

Identifiers

PMID40593769
PMCPMC12215795

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.