ArticleNature communications2025
Closed-loop two-photon functional imaging in a freely moving animal.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Multiple Scales of Coordination along the Body Axis duringThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- A modular multi-color fluorescence microscope for simultaneous tracking of cellular activity and behavior.Nature communications · 2026Article
- High-speed whole-brain imaging in Drosophila.Nature communications · 2026Article
- Short-term memory by distributed neural network oscillators in a simple nervous system.Current biology : CB · 2025Article
- Integrative functional optical imaging approaches: Optics, microfluidics, and machine learning for neuroscience in organoids and small-animal models.Biophysical journal · 2025Review
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Authors and funding
8 authors.
Funding
Abstract
Direct measurement of neural activity in freely moving animals is essential for understanding how the brain controls and represents behaviors. Genetically encoded calcium indicators report neural activity as changes in fluorescence intensity, but brain motion confounds quantitative measurement of fluorescence. Translation, rotation, and deformation of the brain and the movements of intervening scattering or autofluorescent tissue all alter the amount of fluorescent light captured by a microscope. Compared to single-photon approaches, two-photon microscopy is less sensitive to scattering and off-target fluorescence, but more sensitive to motion, and two photon imaging has always required anchoring the microscope to the brain. We developed a closed-loop resonant axial-scanning high-speed two-photon (CRASH2p) microscope for real-time 3D motion correction in unrestrained animals, without implantation of reference markers. We complemented CRASH2p with a 'Pong' scanning strategy and a multi-stage registration pipeline. We performed volumetric ratiometrically corrected functional imaging in the CNS of freely moving Drosophila larvae and discovered previously unknown neural correlates of behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.