Evidence map›Paper›PMID 40593418›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Mammalian Cell Display with Automated Oligo Design and Library Assembly Allows for Rapid Residue Level Conformational Epitope Mapping.

Niklas Berndt Thalén, Maximilian Karlander, Magdalena Malm, Johan Rockberg

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Niklas Berndt ThalénDepartment of Protein Science, KTH-Royal Institute of Technology, Stockholm, Sweden.
Maximilian KarlanderDepartment of Protein Science, KTH-Royal Institute of Technology, Stockholm, Sweden.
Magdalena MalmDepartment of Protein Science, KTH-Royal Institute of Technology, Stockholm, Sweden.
Johan RockbergDepartment of Protein Science, KTH-Royal Institute of Technology, Stockholm, Sweden. johan.rockberg@biotech.kth.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Precise epitope determination of therapeutic antibodies is of great value as it allows for further comprehension of the mechanism of action, therapeutic responsiveness prediction, avoidance of unwanted cross-reactivity, and vaccine design. Alanine scanning mutagenesis can provide an amino acid level resolution of a precise epitope for conformational epitopes. If the mutated antigen is presented on the surface of cells, high throughput screening is enabled without the need for protein expression and purification. Here we present a combinatorial method for rapid mapping of discontinuous epitopes by mammalian antigen display, facilitated by automated workflows for high throughput cloning and tailored software for antigen analysis and oligonucleotide design.

Indexed as

Cell Surface Display TechniquesEpitope MappingEpitopesOligonucleotidesPeptide LibraryAnimalsHumansEpitopesOligonucleotidesPeptide LibraryAlanine scanAutomated oligo designCell surface displayCHO cellsDiscontinuous epitope

Identifiers

PMID40593418

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.