Evidence map›Paper›PMID 40593417›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

High-Density Antigen Microarrays for the Assessment of Antibody Selectivity.

Eni Andersson, Ceke Hellström, Jennie Olofsson, Lovisa Skoglund, Peter Nilsson, Ronald Sjöberg

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eni AnderssonDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
Ceke HellströmDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
Jennie OlofssonDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
Lovisa SkoglundDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
Peter NilssonDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.
Ronald SjöbergDivision of Affinity Proteomics, Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden. ronald.sjoberg@scilifelab.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The success of affinity proteomic methods is dependent on well-characterized affinity binders. Antibodies are the classic affinity binders, and the availability of antibodies is constantly increasing. However, their suitability for a given proteomic method is often context-dependent and requires evaluation of their binding selectivity. One powerful proteomic platform for characterizing the binding selectivity of antibodies is the planar antigen microarray. In this chapter, we describe our in-house developed procedures for generating high-density planar antigen microarrays with an antigen content of 42,100 unique protein fragments. We provide details regarding the assay protocol used for the assessment of the binding selectivity of polyclonal antibodies produced by the Human Protein Atlas.

Indexed as

AntibodiesAntibody SpecificityAntigensProtein Array AnalysisProteomicsHumansProtein BindingAntibodiesAntigensAffinity proteomicsAntibody selectivityAntigen microarraysProtein microarrays

Identifiers

PMID40593417

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.