ArticleMetabolomics : Official journal of the Metabolomic Society2025
Oxygen attachment dissociation (OAD) MS/MS in the identification of positional isomers of dysregulated lipids detected in an ethanol exposure metabolomics study in mice.
Article in Metabolomics : Official journal of the Metabolomic Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Aziridination-Enabled Structural and Quantitative Lipidomics.Journal of the American Society for Mass Spectrometry · 2026Review
- Spatial lipidomics reveals altered lipid profiles in TMEM63A mutant rats with hypomyelination.Scientific reports · 2025Article
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7 authors.
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Abstract
introductionIn metabolic profiling studies the structural characterisation of lipids requires the identification of the head group, carbon number and the position(s) of carbon-carbon double bonds (C = C). Locating the position of double bonds is vital since minor structural differences between positional isomers can alter a lipid's biochemical function.
objectivesOxygen Attachment Dissociation (OAD) is a novel fragmentation technology that enables the localisation of C = C double bonds in lipids. To evaluate its use in the structural characterisation of lipids, OAD has been applied in a discovery-based untargeted analysis of the metabolic impact of acute ethanol exposure in a mouse model.
methodsUHPLC-OAD-MS/MS was used to enhance the identification of lipids found to be significantly altered by acute ethanol exposure in the gut, liver and pancreas tissues of male C57BL/6 mice receiving a Lieber-DeCarli liquid diet either containing 5% ethanol or an isocaloric control diet. Tissue extracts were analysed using untargeted UHPLC-DIA-MS/MS; UHPLC-OAD-MS/MS analysis was performed to further annotate lipids that were significantly increased or diminished in the animals exposed to ethanol.
resultsUHPLC-DIA-MS/MS analysis of gut, liver and pancreas tissue revealed 101 lipids that were significantly increased or diminished in ethanol treated mice. Of the included 83 unsaturated lipids detected, UHPLC-OAD-MS/MS enabled the localisation of C = C double bonds in 61, including isomers indistinguishable by MS/MS with collision induced dissociation.
conclusionsThe results demonstrate the value of OAD-MS/MS in enhancing lipid identification. The resulting improvement may enable better understanding of the underlying biochemistry in the response of mice to exposure to ethanol.
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