Evidence map›Paper›PMID 40592953›Full record

ArticleScientific reports2025

Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes.

Danushka Marapana, Simon A Cobbold, Michal Pasternak, Gerald J Shami, Stuart A Ralph, Sash Lopaticki, Jumana Yousef, Vineet Vaibhav, Laura F Dagley, David Komander and 1 more

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Danushka MarapanaThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia. marapana@wehi.edu.au.
Simon A CobboldThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Michal PasternakThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Gerald J ShamiDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, 3010, Australia.
Stuart A RalphDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, 3010, Australia.
Sash LopatickiThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Jumana YousefThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Vineet VaibhavThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Laura F DagleyThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
David KomanderThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia.
Alan F CowmanThe Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia. cowman@wehi.edu.au.

Funding

National Health and Medical Research Council APP637406Wellcome TrustWellcome Trust 206515_Z_17_Z
6 · The paper itself

Abstract

Ubiquitination is the key eukaryotic post-translational modification that governs protein degradation, localisation, and activity which is mediated by a concerted enzyme cascade. The largest superfamily of these enzymes include the Cullin-RING-Ligase (CRL) complexes. Plasmodium falciparum, the causative agent of the most severe form of malaria in humans, encodes the critical proteins required for ubiquitination, but we do not yet understand the function of this pathway. Here the P. falciparum CRL complexes were characterised to reveal an essential but minimal repertoire controlled by two Cullin scaffolds. A PfCullin1-linked CRL complex, recruiting a single substrate receptor, was identified as being required for parasite inner-membrane biogenesis and DNA replication. A second CRL complex functioning through a PfCullin4 scaffold was identified that utilised a previously unidentified adaptor protein and receptors to support DNA replication. These results show that the P. falciparum CRL complexes are essential in both nuclear maintenance and membrane integrity.

Indexed as

Cullin ProteinsPlasmodium falciparumProtozoan ProteinsDNA ReplicationHumansUbiquitinationCullin ProteinsProtozoan ProteinsCullin-ring-ligaseE3 ligaseMalariaP. falciparumUbiquitination

Identifiers

PMID40592953
PMCPMC12218279

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.