ArticleScientific data2025
A chromosome-scale genome assembly of Giardia duodenalis by long-read sequencing of ten trophozoites.
Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Breaking the annotation barrier: An initial subcellular localization atlas ofMolecular biology of the cell · 2026Article
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10 authors.
Funding
Abstract
Giardia duodenalis, a protozoan parasite of important zoonotic concern, is estimated to cause approximately 280 million human infections annually worldwide. Currently, genome sequencing of G. duodenalis mainly relies on in vitro axenic clones; however, for non-culturable or hard-to-cultivate microorganisms, obtaining sufficient DNA for whole-genome sequencing poses a great challenge. In this study, we isolated ten G. duodenalis trophozoites using single-cell selection technology, followed by the extraction of whole-genome DNA and its amplification via multiple displacement amplification (MDA). The G. duodenalis DNA was sequenced by long-read sequencing (Oxford Nanopore Technologies and Pacific Biosciences), and three main assembly tools (Canu, MECAT2, and RagTag) were used to assemble the sequenced data. As a result, a chromosome-scale genome of G. duodenalis was successfully assembled (assemblage A1 isolate g12a2), with a total genome size of 11.1 Mbp, five contigs, and an N50 value of 3.1 Mbp. This study achieved a chromosome-scale G. duodenalis genome sequencing and assembly from groups of 10 trophozoites, which facilitates protozoan single cell genomics research.
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