ArticleVeterinary research communications2025
Development of TaqMan real-time PCR and droplet digital PCR protocols for the detection of Candidatus Midichloria mitochondrii and evaluation of exposure among wildlife.
Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- "Giant tick" attacks: dynamics of Hyalomma lusitanicum and detection of Rickettsia sibirica mongolitimonae in southern France.Parasite (Paris, France) · 2026Article
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Authors and funding
10 authors.
Funding
Abstract
Candidatus Midichloria mitochondrii (Ca. M. mitochondrii), an endosymbiont intracellular bacterium living in the mitochondria of several tick species, especially ixodid species, has been proposed as a potential marker for monitoring tick-bite exposure. Therefore, the present study aimed to develop two different diagnostic methods, TaqMan-based real-time PCR (rt-PCR) and Droplet Digital PCR (dd-PCR), targeting the 16 S rRNA gene and gyrB gene for the detection of Ca. M. mitochondrii in different wildlife species from several areas of southern Italy. Both techniques were validated using 10-fold serial dilutions of a sequenced positive control up to reach 10
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