Evidence map›Paper›PMID 40590069›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2025

Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle.

Lijun Shi, Zijiao Guo, Nan Shen, Cong Li, Bo Han, Dongxiao Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lijun ShiDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Zijiao GuoDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Nan ShenDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Cong LiDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Bo HanDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
Dongxiao SunDepartment of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.

Funding

2115 Talent Development Program of China Agricultural University 2115National key R & D Program of China 2021YFF1000700National key R & D Program of China 2022YFF1000103Program for Changjiang Scholars and Innovative Research Team in University IRT_15R62
6 · The paper itself

Abstract

Milk fat, an essential component of bovine milk, significantly impacts human health and dairy industry profitability. Our previous research identified that the NFKB2 gene was a key candidate for milk fatty acids (FAs) in dairy cattle. This study aimed to investigate the role of NFKB2 in regulating milk FAs in Holstein cattle through post-GWAS analysis. Knockdown of NFKB2 using siRNA in bovine mammary epithelial cells (BMECs) significantly reduced triglyceride concentrations, lipid droplet secretion, and the expressions of 17 key genes involved in lipid metabolism pathways, including PI3K-AKT, NF-κB, MAPK, adipocytokine, PPAR, and AMPK signaling pathways. These findings highlighted NFKB2 as a critical regulator of milk FA composition through its interactions with key lipid biosynthesis pathways. Further, this study constructed a new Chinese Holstein population including 1065 cows and performed genotyping and milk FAs measurements in these individuals. By SNP identification and association analysis, SNP g.22889812C/T in the 5' flanking region of NFKB2 was significantly associated with multiple milk FAs, including higher C14:1, C17:0, C18:0, and C14 index levels in allele C group, and higher C6:0, C8:0, C14:0, C16:1, and C16 index levels in allele T group. Using computational predictions, luciferase assays, EMSA and super-shift EMSA, we revealed that SNP g.22889812C/T downregulated NFKB2 transcription activity with allele T selectively binding with transcription factor (TF) ZNF282. This study not only advanced the understanding of genetic mechanisms underlying milk fat production but also identified NFKB2 and its causal mutation g.22889812C/T as promising molecular markers for selective breeding to optimize milk fat traits.

Indexed as

Fatty AcidsMilkNF-kappa B p52 SubunitAnimalsCattleFemaleLipid MetabolismPolymorphism, Single NucleotideFatty AcidsNF-kappa B p52 Subunitdairy cattleEMSAfatty acidsknocked‐down of NFKB2transcriptional activity

Identifiers

PMID40590069
PMCPMC12210100

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.