ArticleMedical devices (Auckland, N.Z.)2025
Integration of Portable PCR and a Lateral Flow Assay for the Rapid Detection of HPV Type 16.
Article in Medical devices (Auckland, N.Z.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Deciphering HPV-Associated Immune Evasion in Cervical Cancer Through Multi-Omics Profiling and Computational Screening.Analytical cellular pathology (Amsterdam) · 2026Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Human papillomavirus (HPV) is a leading cause of cervical cancer worldwide, with HPV type 16 accounting for roughly half of cases. Although polymerase chain reaction (PCR) is considered the gold standard for HPV detection, its reliance on gel electrophoresis can be costly and require specialized facilities. Objective: We developed a diagnostic approach integrating PCR with a lateral flow assay to detect HPV type 16 using gold nanoparticles as visible labels. Methods: Primers targeting the L1 gene of HPV 16 were labeled with 6FAM (forward) and biotin (reverse). Amplified products were applied to lateral flow strips preloaded with streptavidin-gold nanoparticle conjugates. Visible red bands on the test and control lines indicated successful detection. Results: The optimized assay produced a clear band at ~333 bp by gel electrophoresis and yielded distinct red lines on the lateral flow strip for positive samples. Compared to electrophoresis, this format has a faster turnaround and can reduce costs by eliminating bulky equipment. Conclusion: This simplified and cost-effective method provides a user-friendly alternative to traditional electrophoresis, making it suitable for resource-limited settings. Further large-scale clinical validation is warranted, including cost analyses and multiplexing for additional genotypes.
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