Evidence map›Paper›PMID 40589943›Full record

ArticleThe Yale journal of biology and medicine2025

For All the Primate FANS: Optimized Isolation of Nuclei from Frozen Postmortem Primate Brain for Fluorescence-Assisted Nuclei Sorting (FANS).

Elaine E Guevara, Begün Erbaba, Gregory M Cresswell, Melissa K Edler, Chet C Sherwood

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Yale journal of biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Elaine E GuevaraDepartment of Evolutionary Anthropology, Duke University, Durham, NC, USA.
Begün ErbabaDepartment of Anthropology and Center for the Advanced Study of Human Paleobiology, The George Washington University, Washington DC, USA.
Gregory M CresswellSchool of Medicine and Health Sciences, The George Washington University, Washington DC, USA.
Melissa K EdlerDepartment of Anthropology, School of Biomedical Sciences, and Brain Health Research Institute, Kent State University, Kent, OH, USA.
Chet C SherwoodDepartment of Anthropology and Center for the Advanced Study of Human Paleobiology, The George Washington University, Washington DC, USA.

Funding

Alzheimer's Disease Pathology in a Primate ModelR01AG067419 · NIA · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI HOPKINS, WILLIAM D · 2021 to 2025
$3.8M
Lifespan and Cardiovascular Health Effects on The Brain in Humans and Great ApeR01AG087945 · NIA · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Melissa K. Edler, PATRICK R HOF · 2024 to 2026
$2.9M
Comparative Epigenomics of Primate BrainsR01HG011641 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI KONOPKA, GENEVIEVE, SHERWOOD, CHESTER · 2021 to 2024
$2.4M
NHGRI NIH HHS R01 HG011641NIA NIH HHS R01 AG067419NIA NIH HHS R01 AG087945
6 · The paper itself

Abstract

Epigenetic alterations are cell type-specific and require methods like single cell sequencing and cell type sorting by flow cytometry. These methods often rely on the availability of fresh tissue, yet postmortem frozen tissue is typically the only material available from non-experimental subjects, including humans and other nonhuman primates (NHP). Many insights can be gained from analysis of these precious samples. To this end, we developed a protocol for isolating intact nuclei from small starting amounts of postmortem frozen chimpanzee (

Indexed as

BrainCell NucleusFlow CytometryAnimalsAutopsyFreezingHumansPan troglodytesPrimateschimpanzeecortexflow sortingmethylationnonhuman primatesingle cell genomics

Identifiers

PMID40589943
PMCPMC12204041

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.