ArticleFrontiers in microbiology2025
Intracellular self-assembly and metabolite analysis of key enzymes for L-lysine synthesis based on key components of cellulosomes.
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- CRISPR-enhanced microalgae biosynthesis: a promising approach for future functional feed ingredients.Journal of animal science and biotechnology · 2026Review
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11 authors.
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Abstract
Introduction: Cellulosome is a natural multi-enzyme complex in the extracellular space of anaerobic microorganisms, which has the advantages of small molecular weight, multiple binding sites, and strong designability. This study aimed to explore the influence of intracellular self-assembly complexes on L-lysine biosynthesis. Methods: Two novel L-lysine-engineered bacteria modification strategies were designed, considering the L-lysine biosynthesis pathway using DocA-S3/Coh as an efficient intracellular assembly element: pairwise assembly of key enzymes in cells and multi-enzyme assembly based on scaffolding proteins. Seven strains of key enzyme pairwise-assembled engineered bacteria were constructed, and four strains of multi-enzyme-assembled engineered bacteria were designed based on the scaffold protein genome. Results: The production of L-lysine by multi-enzyme-assembled engineered strain Conclusion: This strategy exerts the "proximity effect" among multi-enzyme complexes, improves the transfer efficiency of intermediate metabolites between different catalytic active centers, indirectly improves the catalytic rate of each key enzyme, and provides a novel idea and technical platform for other multi-enzyme intracellular assemblies.
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