ArticleVirology journal2025
Enhanced detection of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) antibodies in various animal species using oligomeric nucleocapsid protein-based diagnostics.
Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Establishment of a rapid detection method for severe fever with thrombocytopenia syndrome virus based on reverse transcription-recombinase polymerase amplification with lateral flow nucleic acid chromatography assay technology.Frontiers in microbiology · 2026Article
- Recent Advances in Lateral Flow Immunoassay for Rapid Diagnosis of Viral Diseases.Transboundary and emerging diseases · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) is a zoonotic pathogen that affects various animal species, often asymptomatically, while causing fatal disease in humans. Current serological tests are limited, which led to the development of novel diagnostic assays targeting the viral nucleoprotein (NP), a conserved and immunodominant protein across SFTSV strains. This study validated the new diagnostics using field monkey sera, employing Enzyme-Linked Immunosorbent Assay (ELISA) with infected cell lysate (ICL-ELISA) as the antigen. Immunoblotting with recombinant NP (rNP) demonstrated 94.6% specificity and 96.3% sensitivity for anti-NP antibody detection. As NP exists in both monomeric and RNA-binding oligomeric forms, rNP was expressed in both configurations and used for ELISA and rapid lateral flow assays (LFA). The oligomeric rNP exhibited superior antigenicity, achieving 97.3% specificity and 92.6% sensitivity, outperforming ICL-ELISA. Additionally, oligomer-enriched rNP was applied to develop an LFA test strip, where rNP conjugated with magnetic nanoparticles enhanced immunocomplex enrichment and clarity. The NP Ab-LFA test achieved 84.2% specificity and 100% sensitivity, successfully detecting anti-NP antibodies in rabbit, mouse, and monkey sera. These innovative diagnostic tools offer reliable methods for detecting SFTSV antibodies, facilitating disease control and mitigating the risk of human outbreaks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.