Evidence map›Paper›PMID 40588067›Full record

ArticleInternational journal of radiation oncology, biology, physics2025

A Feasibility Study of Preclinical Ocular X-Ray FLASH Radiation Therapy.

Devin Miles, Daniel Sforza, Marisol Cano, Cornelia Peterson, Kathleen Gabrielson, John W Wong, James Handa, Mohammad Rezaee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of radiation oncology, biology, physics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Devin MilesDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland. Electronic address: dmiles18@jh.edu.
Daniel SforzaDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Marisol CanoDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Cornelia PetersonDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Comparative Pathobiology, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts.
Kathleen GabrielsonDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
John W WongDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland.
James HandaDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Mohammad RezaeeDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland. Electronic address: mrezaee1@jhmi.edu.

Funding

An ultra-high (FLASH) dose rate x-ray cabinet system for pre-clinical laboratory radiation researchR01CA262097 · NCI · JOHNS HOPKINS UNIVERSITY · PI REZAEE, MOHAMMAD · 2021 to 2024
$2.1M
NCI NIH HHS R01 CA262097
6 · The paper itself

Abstract

purposeUltrahigh-dose-rate radiation therapy (FLASH RT) has not previously been studied for the treatment of ocular targets. This work describes the feasibility of ocular FLASH irradiation of a single mouse eye using kilovoltage x-ray sources with rotating anode technology. METHODS AND MATERIALS: A 75-kW-powered rotating anode kilovoltage x-ray source was implemented for FLASH and conventional (CONV) dose-rate irradiation of superficial targets in small animals. A docking immobilization device was designed to facilitate irradiation of an individual mouse eye. Mouse positioning reproducibility was assessed via Hausdorff distance and Dice similarity coefficients of features from cone beam computed tomographies (CT) of immobilized animals. Thermoluminescent dosimeters and Gafchromic film dosimeters were used to characterize output (dose rate), depth dose rate, and to assess geometric parameters of the beam. In a pilot study of ocular irradiation, healthy C57BL/6J mouse eyes were irradiated to doses of up to 26 Gy at either FLASH or CONV dose rates. Retinal function was assessed 2 months following irradiation using electroretinography. Morphologic changes were evaluated via histopathology.

resultsAnimal setup was highly reproducible, with typical Hausdorff distances between bony features of 0.34 mm and Dice similarity coefficient of 0.92 for surface features. At FLASH irradiation settings, dose rates of 52.6 ± 2.6 Gy/s were measured using in vivo thermoluminescent dosimeters. CONV dose rates of 1.1 ± 0.1 Gy/s were achievable with the same setup by minimizing the input current. Following irradiation to 26 Gy, retinal function was preserved for FLASH-irradiated eyes but significantly impaired in CONV-irradiated eyes. Histopathology assessment confirmed significant inner retinal atrophy in CONV-irradiated eyes that was absent in FLASH-irradiated eyes.

conclusionsIrradiation of the mouse eye was feasible at FLASH and CONV dose rates by the orthovoltage x-ray using the rotating anode x-ray source. A pilot study of ocular irradiation in healthy mice demonstrated sparing effects of FLASH on the mouse retina.

Indexed as

EyeRetinaAnimalsCone-Beam Computed TomographyElectroretinographyFeasibility StudiesMiceMice, Inbred C57BLPilot ProjectsRadiotherapy DosageReproducibility of ResultsThermoluminescent Dosimetry

Identifiers

PMID40588067
PMCPMC12314887

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.