ArticleJournal of advanced research2026
Upregulation of a cytochrome P450 gene, CYP6B50, confers multi-insecticide resistance in Spodoptera frugiperda.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Coordinated regulation of glutathione S-transferases confers metabolic flexibility in multi-insecticide-resistant Frankliniella occidentalis (Pergande).Journal of advanced research · 2026Article
- Variation in Label-Rate Insecticide Efficacy and Control Failure Risk Among Brazilian Populations ofInsects · 2026Article
- Mechanisms and Genetic Drivers of Resistance of Insect Pests to Insecticides and Approaches to Its Control.Toxics · 2025Review
- Cooperative microbial metabolism enhances tryptophan-mediated insecticide detoxification in the fall armyworm.The ISME journal · 2025Article
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9 authors.
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Abstract
introductionThe fall armyworm, Spodoptera frugiperda, is a globally invasive pest that has developed resistance to numerous insecticides. Cytochrome P450 enzymes have been commonly implicated in insecticide resistance in insect pests, including in S. frugiperda.
objectiveOur previous study revealed that the P450 gene CYP6B50 was significantly overexpressed in a multi-insecticide resistant strain of S. frugiperda. However, the functional role of CYP6B50 in conferring multiple resistance remains unclear.
methodsHere we used reverse genetic (RNAi, CRISPR/Cas9, and transgenic Drosophila melanogaster) and computational modelling approaches to investigate the role of CYP6B50 in resistance to insecticides.
resultsFunctional analysis revealed that CYP6B50 is highly expressed during the larval stage of S. frugiperda, particularly in key tissues associated with detoxification such as the fat body, midgut, hemolymph, and Malpighian tubules. The expression of CYP6B50 was significantly upregulated after exposure to the insecticides indoxacarb, chlorantraniliprole, lambda-cyhalothrin, tetrachlorantraniliprole, and deltamethrin. Functional validation of CYP6B50 confirmed its contribution to resistance against the five tested insecticides. Molecular docking revealed a strong binding affinity of CYP6B50 to these insecticides.
conclusionsThese results identify CYP6B50 as a key generalist detoxification P450 in S. frugiperda and its role in mediating resistance to multiple insecticides. These findings provide insights into the role of cytochrome P450s in the evolution of insecticide resistance and will inform the development of strategies to manage or overcome resistance in a globally distributed crop pest.
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