Evidence map›Paper›PMID 40587785›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.

Julie L McDonald, Nathan P Shapiro, Amanuella A Mengiste, Sarah Kaines, Spencer M Whitney, Robert H Wilson, Matthew D Shoulders

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. Article
4 · The record

Corrections and comments

  • Update of
    2025
5 · Who and what money

Authors and funding

7 authors.

Julie L McDonaldDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.ORCID 0000-0002-3715-9619
Nathan P ShapiroDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Amanuella A MengisteDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Sarah KainesPlant Science Division, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia.ORCID 0000-0002-1875-0024
Spencer M WhitneyPlant Science Division, Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia.ORCID 0000-0003-2954-2359
Robert H WilsonDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
Matthew D ShouldersDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.ORCID 0000-0002-6511-3431

Funding

Leveraging Next-Generation Directed Evolution Platforms and Chemical Control of Proteostasis to Deliver Robust Biotechnologies and Illuminate Roles of Chaperone Networks in Protein EvolutionR35GM136354 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SHOULDERS, MATTHEW DONALD · 2020 to 2024
$2.1M
BroadIgnite Fellowship Graduate FellowshipHHS | NIH | National Institute of General Medical Sciences (NIGMS) 1R35GM136354Martin Family Society Fellowship for Sustainability Graduate FellowshipMIT | Abdul Latif Jameel Water and Food Systems Lab, Massachusetts Institute of Technology (J-WAFS) Grand Challenge AwardMIT Future of Science Fellowship Graduate FellowshipMIT Robert J. Sibley Fellowship Graduate FellowshipNIGMS NIH HHS R35 GM136354NSF (NSF) 2244770
6 · The paper itself

Abstract

Carbon dioxide (CO

Indexed as

Directed Molecular EvolutionOxygenRibulose-Bisphosphate CarboxylaseCarbon DioxideKineticsPhotosynthesisCarbon DioxideOxygenRibulose-Bisphosphate Carboxylasecarbon fixationdirected evolutionenzyme engineeringMutaT7Ribulose‐1,5‐bisphosphate Carboxylase/Oxygenase (Rubisco)

Identifiers

PMID40587785
PMCPMC12260525

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.